A guide to fluorescent protein FRET pairs

BT Bajar, ES Wang, S Zhang, MZ Lin, J Chu - Sensors, 2016 - mdpi.com
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

K Joron, JO Viegas, L Haas-Neill, S Bier, P Drori… - Nature …, 2023 - nature.com
Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …

Single-photon microscopy to study biomolecular condensates

E Perego, S Zappone, F Castagnetti, D Mariani… - Nature …, 2023 - nature.com
Biomolecular condensates serve as membrane-less compartments within cells,
concentrating proteins and nucleic acids to facilitate precise spatial and temporal …

Structural and photophysical characterization of the small ultra-red fluorescent protein

A Maiti, CZ Buffalo, S Saurabh… - Nature …, 2023 - nature.com
Abstract The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of
fluorescent protein with exceptional photostability and brightness derived from …

Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence

LM Barnett, TE Hughes, M Drobizhev - PloS one, 2017 - journals.plos.org
The goal of this work is to determine how GCaMP6m's fluorescence is altered in response to
Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how …

Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging

DK Tuchina, IG Meerovich, OA Sindeeva… - Journal of …, 2020 - Wiley Online Library
Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low
molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has …

Chemical sectioning fluorescence tomography: high-throughput, high-contrast, multicolor, whole-brain imaging at subcellular resolution

X Wang, H Xiong, Y Liu, T Yang, A Li, F Huang, F Yin… - Cell Reports, 2021 - cell.com
A thorough neuroanatomical study of the brain architecture is crucial for understanding its
cellular compositions, connections, and working mechanisms. However, the fine-and …

RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections

B Lace, C Su, DI Perez, M Rodriguez-Franco, T Vernié… - Elife, 2023 - elifesciences.org
Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the
establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs …

Quantitative imaging of genetically encoded fluorescence lifetime biosensors

CQ Vu, S Arai - Biosensors, 2023 - mdpi.com
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for
quantitative imaging, enabling precise measurement of cellular metabolites, molecular …