A guide to fluorescent protein FRET pairs
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
Photoinduced chemistry in fluorescent proteins: curse or blessing?
A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …
Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation
K Joron, JO Viegas, L Haas-Neill, S Bier, P Drori… - Nature …, 2023 - nature.com
Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …
Single-photon microscopy to study biomolecular condensates
Biomolecular condensates serve as membrane-less compartments within cells,
concentrating proteins and nucleic acids to facilitate precise spatial and temporal …
concentrating proteins and nucleic acids to facilitate precise spatial and temporal …
Structural and photophysical characterization of the small ultra-red fluorescent protein
Abstract The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of
fluorescent protein with exceptional photostability and brightness derived from …
fluorescent protein with exceptional photostability and brightness derived from …
Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence
The goal of this work is to determine how GCaMP6m's fluorescence is altered in response to
Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how …
Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how …
Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging
DK Tuchina, IG Meerovich, OA Sindeeva… - Journal of …, 2020 - Wiley Online Library
Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low
molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has …
molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has …
Chemical sectioning fluorescence tomography: high-throughput, high-contrast, multicolor, whole-brain imaging at subcellular resolution
A thorough neuroanatomical study of the brain architecture is crucial for understanding its
cellular compositions, connections, and working mechanisms. However, the fine-and …
cellular compositions, connections, and working mechanisms. However, the fine-and …
RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections
B Lace, C Su, DI Perez, M Rodriguez-Franco, T Vernié… - Elife, 2023 - elifesciences.org
Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the
establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs …
establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs …
Quantitative imaging of genetically encoded fluorescence lifetime biosensors
CQ Vu, S Arai - Biosensors, 2023 - mdpi.com
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for
quantitative imaging, enabling precise measurement of cellular metabolites, molecular …
quantitative imaging, enabling precise measurement of cellular metabolites, molecular …