The covalent immobilisation of enzymes generally involves the use of highly reactive
crosslinkers, such as glutaraldehyde, to couple enzyme molecules to each other or to …

The way to immobilize a protein onto a solid surface is the cornerstone for developing many
bioanalytical assays which are applied in protein functional assay, protein-ligand interaction …

Protein immobilization is particularly significant in proteomics, interactomics, and in vitro
drug screening. It is an essential primary step for numerous biological techniques that rely …

Allosteric ligands are promising drugs owing to their remote regulations of the orthosteric
ligand signaling pathway. There are few allosteric ligands due to the lack of handy and …

The design, synthesis, and discovery of dual-target compounds are considered as a
promising strategy to develop new drugs with improved safety and efficacy compared with …

Surface-enhanced Raman spectroscopy (SERS) is an ultrasensitive technique for both
detection and structural characterizations. To further exploit these advantages, we designed …

The platforms based on immobilization of transmembrane proteins have become an
effective way to study drug-protein interaction and identify new leads for drug discovery …

Highlights•This review summarizes the immobilization methods of receptor
chromatography.•The applications of receptor chromatography are discussed.•Future …

Accurate orientations and stable conformations of membrane receptor immobilization are
particularly imperative for accurate drug screening and ligand–protein affinity analysis …

During integration to the solid surface, the effects of tags introduced for bioorthogonal
reactions on protein activity have received far less investigation. This represents the major …