Advances in the application of genetic manipulation methods to apicomplexan parasites

CE Suarez, RP Bishop, HF Alzan, WA Poole… - International journal for …, 2017 - Elsevier
Apicomplexan parasites such as Babesia, Theileria, Eimeria, Cryptosporidium and
Toxoplasma greatly impact animal health globally, and improved, cost-effective measures to …

Recent Advances in Molecular Genetic Tools for Babesia

H Hakimi, M Asada, S Kawazu - Veterinary Sciences, 2021 - mdpi.com
Development of in vitro culture and completion of genome sequencing of several Babesia
parasites promoted the efforts to establish transfection systems for these parasites to dissect …

Comparative analysis of gene expression between Babesia bovis blood stages and kinetes allowed by improved genome annotation

MW Ueti, WC Johnson, LS Kappmeyer… - International Journal for …, 2021 - Elsevier
Throughout their life cycle, Babesia parasites alternate between a mammalian host, where
they cause babesiosis, and the tick vector. Transition between hosts results in distinct …

Establishment of a stable transfection and gene targeting system in Babesia divergens

EFG Cubillos, P Snebergerova, S Borsodi… - Frontiers in Cellular …, 2023 - frontiersin.org
Babesia divergens is an emerging tick-borne pathogen considered as the principal
causative agent of bovine babesiosis in Europe with a notable zoonotic risk to human …

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

S Wang, D Li, F Chen, W Jiang, W Luo… - Frontiers in Cellular …, 2022 - frontiersin.org
Genetic modification provides an invaluable molecular tool to dissect the biology and
pathogenesis of pathogens. However, no report is available about the genetic modification …

Novel Babesia bovis exported proteins that modify properties of infected red blood cells

H Hakimi, TJ Templeton, M Sakaguchi… - PLoS …, 2020 - journals.plos.org
Babesia bovis causes a pathogenic form of babesiosis in cattle. Following invasion of red
blood cells (RBCs) the parasite extensively modifies host cell structural and mechanical …

Genome Editing of Babesia bovis Using the CRISPR/Cas9 System

H Hakimi, T Ishizaki, Y Kegawa, O Kaneko, S Kawazu… - MSphere, 2019 - Am Soc Microbiol
Babesia bovis, the most virulent causative agent of bovine babesiosis, is prevalent in
tropical and subtropical regions of the world. Although the whole-genome sequence was …

Establishment of transient and stable transfection systems for Babesia ovata

H Hakimi, J Yamagishi, Y Kegawa, O Kaneko… - Parasites & vectors, 2016 - Springer
Background Bovine babesiosis is a tick-borne disease caused by several species of
Babesia which produce acute and fatal disease in cattle and affect livestock industry …

Critical role of Babesia bovis spherical body protein 3 in ridge formation on infected red blood cells

A Fathi, H Hakimi, M Sakaguchi, J Yamagishi… - PLoS …, 2024 - journals.plos.org
Babesia bovis, an apicomplexan intraerythrocytic protozoan parasite, causes serious
economic loss to cattle industries around the world. Infection with this parasite leads to …

To kill a piroplasm: genetic technologies to advance drug discovery and target identification in Babesia

CD Keroack, B Elsworth, MT Duraisingh - International journal for …, 2019 - Elsevier
Babesia parasites infect a diverse range of vertebrate hosts, from penguins to pigs.
Recently, the emergence of zoonotic Babesia infection has been increasing, and the list of …