Plant DNA barcoding: from gene to genome
ABSTRACT DNA barcoding is currently a widely used and effective tool that enables rapid
and accurate identification of plant species; however, none of the available loci work across …
and accurate identification of plant species; however, none of the available loci work across …
[HTML][HTML] Choosing and using a plant DNA barcode
The main aim of DNA barcoding is to establish a shared community resource of DNA
sequences that can be used for organismal identification and taxonomic clarification. This …
sequences that can be used for organismal identification and taxonomic clarification. This …
[HTML][HTML] Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …
second largest group of organisms based on global richness estimates, with around 3 …
A universal probe set for targeted sequencing of 353 nuclear genes from any flowering plant designed using k-medoids clustering
Sequencing of target-enriched libraries is an efficient and cost-effective method for obtaining
DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the …
DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the …
[HTML][HTML] Phylogenies of the 16S rRNA gene and its hypervariable regions lack concordance with core genome phylogenies
HB Hassler, B Probert, C Moore, E Lawson… - Microbiome, 2022 - Springer
Abstract Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in
species delineation, and now widely in microbiome studies. However, the gene suffers from …
species delineation, and now widely in microbiome studies. However, the gene suffers from …
Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity
The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly
used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been …
used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been …
Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants
China Plant BOL Group 1, DZ Li… - Proceedings of the …, 2011 - National Acad Sciences
A two-marker combination of plastid rbcL and matK has previously been recommended as
the core plant barcode, to be supplemented with additional markers such as plastid trnH …
the core plant barcode, to be supplemented with additional markers such as plastid trnH …
Navigating the tip of the genomic iceberg: Next‐generation sequencing for plant systematics
• Premise of the study: Just as Sanger sequencing did more than 20 years ago, next‐
generation sequencing (NGS) is poised to revolutionize plant systematics. By combining …
generation sequencing (NGS) is poised to revolutionize plant systematics. By combining …
[HTML][HTML] Use of ITS2 region as the universal DNA barcode for plants and animals
H Yao, J Song, C Liu, K Luo, J Han, Y Li, X Pang, H Xu… - PloS one, 2010 - journals.plos.org
Background The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is
regarded as one of the candidate DNA barcodes because it possesses a number of …
regarded as one of the candidate DNA barcodes because it possesses a number of …
Use of DNA barcodes to identify flowering plants
Methods for identifying species by using short orthologous DNA sequences, known as “DNA
barcodes,” have been proposed and initiated to facilitate biodiversity studies, identify …
barcodes,” have been proposed and initiated to facilitate biodiversity studies, identify …