Recent advances in droplet microfluidics for single-cell analysis

Z Jiang, H Shi, X Tang, J Qin - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Single-cell manipulation and analysis is essential for studying many fundamental biological
processes and discovering cellular heterogeneity. Multi-parameter studies of intercellular …

Recent progress in high-throughput droplet screening and sorting for bioanalysis

G Sun, L Qu, F Azi, Y Liu, J Li, X Lv, G Du… - Biosensors and …, 2023 - Elsevier
Owing to its ability to isolate single cells and perform high-throughput sorting, droplet sorting
has been widely applied in several research fields. Compared with flow cytometry, droplet …

Droplet microfluidics: from proof-of-concept to real-world utility?

A Suea-Ngam, PD Howes, M Srisa-Art… - Chemical …, 2019 - pubs.rsc.org
Droplet microfluidics constitutes a diverse and practical tool set that enables chemical and
biological experiments to be performed at high speed and with enhanced efficiency when …

Image-based single cell sorting automation in droplet microfluidics

M Sesen, G Whyte - Scientific reports, 2020 - nature.com
The recent boom in single-cell omics has brought researchers one step closer to
understanding the biological mechanisms associated with cell heterogeneity. Rare cells that …

UV–Vis spectra-activated droplet sorting for label-free chemical identification and collection of droplets

TA Duncombe, A Ponti, FP Seebeck… - Analytical …, 2021 - ACS Publications
We introduce the UV–vis spectra-activated droplet sorter (UVADS) for high-throughput label-
free chemical identification and enzyme screening. In contrast to previous absorbance …

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

V Anagnostidis, B Sherlock, J Metz, P Mair… - Lab on a Chip, 2020 - pubs.rsc.org
Uncovering the heterogeneity of cellular populations and multicellular constructs is a long-
standing goal in fields ranging from antimicrobial resistance to cancer research. Emerging …

Recent advances on sorting methods of high-throughput droplet-based microfluidics in enzyme directed evolution

X Fu, Y Zhang, Q Xu, X Sun, F Meng - Frontiers in Chemistry, 2021 - frontiersin.org
Droplet-based microfluidics has been widely applied in enzyme directed evolution (DE), in
either cell or cell-free system, due to its low cost and high throughput. As the isolation …

Droplet microfluidics for CTC-based liquid biopsy: a review

L Jiang, H Yang, W Cheng, Z Ni, N Xiang - Analyst, 2023 - pubs.rsc.org
Circulating tumor cells (CTCs) are important biomarkers of liquid biopsy. The number and
heterogeneity of CTCs play an important role in cancer diagnosis and personalized …

Droplet microfluidics and directed evolution of enzymes: an intertwined journey

A Stucki, J Vallapurackal, TR Ward… - Angewandte Chemie …, 2021 - Wiley Online Library
Evolution is essential to the generation of complexity and ultimately life. It relies on the
propagation of the properties, traits, and characteristics that allow an organism to survive in …

Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …