The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Abstract The RNA polymerase II (Pol II) pre-initiation complex (PIC) is a critical node in
eukaryotic transcription regulation, and its formation is the major rate-limiting step in …

Homotypic chromatin interactions and loop extrusion are thought to be the two main drivers
of mammalian chromosome folding. Here we tested the role of RNA polymerase II (RNAPII) …

The spatiotemporal control of gene expression is dependent on the activity of cis-acting
regulatory sequences, called enhancers, which regulate target genes over variable genomic …

The Polycomb system has fundamental roles in regulating gene expression during
mammalian development. However, how it controls transcription to enable gene repression …

Few transcription factors have been examined for their direct roles in physically connecting
enhancers and promoters. Here acute degradation of Yin Yang 1 (YY1) in erythroid cells …

Enhancer-mediated gene activation generally requires physical proximity between
enhancers and their target gene promoters. However, the molecular mechanisms by which …

Enhancers are cis-regulatory elements that can activate transcription remotely to regulate a
specific pattern of a gene's expression. Genes typically have many enhancers that are often …

Distinct clusters of RNA polymerase II are responsible for gene transcription inside
eukaryotic cell nuclei. Despite the functional implications of such subnuclear organization …

Temporal and spatial specificity of gene expression is highly regulated through a rich milieu
of regulatory DNAs embedded in the genome. Enhancers represent a major class of …