Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

Bacteria of the phylum Planctomycetes have been previously reported to possess several
features that are typical of eukaryotes, such as cytosolic compartmentalization and …

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by
a lipid bilayer from which spike (S) protein trimers protrude. Heavily glycosylated S trimers …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …

The endoplasmic reticulum and mitochondria are main hubs of eukaryotic membrane
biogenesis that rely on lipid exchange via membrane contact sites,–, but the underpinning …

Ribosomes translate genetic information into primary structure. During translation, various
cofactors transiently bind to the ribosome that undergoes prominent conformational and …

The proteasome is a major proteolytic machine that regulates cellular proteostasis through
selective degradation of ubiquitylated proteins,. A number of ubiquitin-related molecules …

Structural biology studies performed inside cells can capture molecular machines in action
within their native context. In this work, we developed an integrative in-cell structural …