Bacteria are simple and cost effective hosts for producing recombinant proteins. However,
their physiological features may limit their use for obtaining in native form proteins of some …

The microorganism Escherichia coli is commonly used for recombinant protein production.
Despite several advantageous characteristics like fast growth and high protein yields, its …

Gram negative bacteria possess a large variety of protein transport systems, by which
proteins that are synthesised in the cytosol are exported to destinations in the cell envelope …

Correct protein function depends on delivery to the appropriate cellular or subcellular
compartment. Following the initiation of protein synthesis in the cytosol, many bacterial and …

Nascent polypeptides emerging from the ribosome are assisted by a pool of molecular
chaperones and targeting factors, which enable them to efficiently partition as cytosolic …

A widespread feature in the genomes of most bacteria and archaea is an array of clustered,
regularly interspaced short palindromic repeats (CRISPRs) that, together with a group of …

Despite the fundamental importance of E. coli in the manufacture of a wide range of
biotechnological and biomedical products, extensive process and/or target optimisation is …

The Tat (twin-arginine transport) pathway is a protein-targeting system dedicated to the
transmembrane translocation of fully folded proteins. This system is highly prevalent in the …

Co-translational protein targeting by the Signal Recognition Particle (SRP) is an essential
cellular pathway that couples the synthesis of nascent proteins to their proper cellular …

Twin-arginine translocation (Tat) denotes a protein transport pathway in bacteria, archaea
and plant chloroplasts, which is specific for precursor proteins harbouring a characteristic …