Shifting from chemical to biotechnological processes is one of the cornerstones of 21st
century industry. The production of a great range of chemicals via biotechnological means is …

Increasing global energy demand and environmental concerns associated with petroleum
have raised interest in biofuels for reducing dependency on crude oil and promote carbon …

Rational engineering of metabolism is important for bio-production using microorganisms.
Metabolic design based on in silico simulations and experimental validation of the metabolic …

We focus on the application of constraint-based methodologies and, more specifically, flux
balance analysis in the field of metabolic engineering, and enumerate recent developments …

Background The sustainable production of biofuels remains one of the major issues of the
upcoming years. Among the number of most desirable molecules to be produced, butanol …

Abstract Background 3-hydroxypropionic acid (3HP) is an important chemical precursor for
the production of bioplastics. Microbial production of 3HP from glycerol has previously been …

Motivation: Although constraint-based flux analysis of knockout strains has facilitated the
production of desirable metabolites in microbes, current screening methods have placed a …

Gene deletion strategies using flux balance analysis (FBA) have improved the growth‐
coupled production of various compounds. However, the productivities were often below the …

In the context of the global objective of shifting from petroleum to a biomass‐based
economy, the research on fermentative strategies to produce alternative biofuels and …

Microorganisms are widely used to produce valuable compounds. Because thousands of
metabolic reactions occur simultaneously and many metabolic reactions are related to target …