Fusion proteins play an essential role in the biosciences but suffer from several key
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …

Highlights•Dha in proteins is easily and flexibly accessible under mild conditions.•Dha
reactivity is used in β, γ-C–S/Se/N/C bond-formation and allows 'zero scar'protein …

Post-translational modifications with ubiquitin (Ub) and ubiquitin-like proteins (Ubls) are
regulated by isopeptidases termed deubiquitinases (DUBs) and Ubl proteases. Here, we …

The post-translational modification of proteins with ubiquitin (Ub) and Ub-like modifiers
(Ubls) represents one of the most important regulators in eukaryotic biology. Polymeric …

Post-translational modification of proteins with ubiquitin and ubiquitin-like proteins (Ubls) is
central to the regulation of eukaryotic cellular processes. Our ability to study the effects of …

Nature has developed a plethora of protein machinery to operate and maintain nearly every
task of cellular life. These processes are tightly regulated via post‐expression modifications …

Conspectus Chemical manipulation of naturally occurring peptides offers a convenient route
for generating analogs to screen against different therapeutic targets. However, the limited …

Organic chemistry allows for the modification and chemical preparation of protein analogues
for various studies. The thiolate side chain of the Cys residue has been a key functionality in …

Chemical ubiquitination is an effective approach for accessing structurally defined, atypical
ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a …

Enzymes are powerful tools for protein labelling due to their specificity and mild reaction
conditions. Many protocols, however, are restricted to modifications at protein termini, rely on …