Recent advances and promises in nitrile hydratase: from mechanism to industrial applications
Nitrile hydratase (NHase, EC 4.2. 1.84) is one type of metalloenzyme participating in the
biotransformation of nitriles into amides. Given its catalytic specificity in amide production …
biotransformation of nitriles into amides. Given its catalytic specificity in amide production …
Nitrile-converting enzymes as a tool to improve biocatalysis in organic synthesis: recent insights and promises
JS Gong, JS Shi, ZM Lu, H Li, ZM Zhou… - Critical reviews in …, 2017 - Taylor & Francis
Nitrile-converting enzymes, including nitrilase and nitrile hydratase (NHase), have received
increasing attention from researchers of industrial biocatalysis because of their critical role …
increasing attention from researchers of industrial biocatalysis because of their critical role …
Cyanide-free synthesis of aromatic nitriles from aldoximes: discovery and application of a novel heme-containing aldoxime dehydratase
Z Chen, F Mao, H Zheng, Q Xiao, Z Ding… - Enzyme and Microbial …, 2021 - Elsevier
Aromatic nitriles are important structural motifs that frequently existed in pharmaceutical
drugs. Due to the convenient synthesis of aldoximes from aldehydes, the dehydration of …
drugs. Due to the convenient synthesis of aldoximes from aldehydes, the dehydration of …
Formation of cross-linked nitrile hydratase aggregates in the pores of tannic-acid-templated magnetic mesoporous silica: characterization and catalytic application
J Gao, H Yu, L Zhou, Y He, L Ma, Y Jiang - Biochemical Engineering …, 2017 - Elsevier
Tannic-acid-templated magnetic mesoporous silica nanoparticles (TA-MMSNs) were
synthesized for the first time. The TA-MMSNs were monodisperse spherical particles with a …
synthesized for the first time. The TA-MMSNs were monodisperse spherical particles with a …
Nitrile hydratases: from industrial application to acetamiprid and thiacloprid degradation
S Sun, J Zhou, J Jiang, Y Dai… - Journal of Agricultural and …, 2021 - ACS Publications
The widespread application of neonicotinoid insecticides (NEOs) in agriculture causes a
series of environmental and ecological problems. Microbial remediation is a popular …
series of environmental and ecological problems. Microbial remediation is a popular …
Genome mining for enzyme discovery
Nature appears as the veteran protein engineer since it began its bioengineering
'experiments' billions of years ago. 1 A number of strategies have been developed to exploit …
'experiments' billions of years ago. 1 A number of strategies have been developed to exploit …
Engineering of the thermophilic nitrile hydratase from Pseudonocardia thermophila JCM3095 for large-scale nicotinamide production based on sequence-activity …
Z Zhou, D Ma, Z Cheng - International Journal of Biological Macromolecules, 2021 - Elsevier
The green biocatalyst nitrile hydratase (NHase) is able to bio-transform 3-cyanopyridine into
nicotinamide. As the NHase reaction is exothermic, an enzyme with high activity and stability …
nicotinamide. As the NHase reaction is exothermic, an enzyme with high activity and stability …
Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining
J Wang, P Cheng, Y Wu, A Wang, F Liu, X Pei - Journal of biotechnology, 2019 - Elsevier
Abstract (R)-Pantolactone (PL) is a key chiral intermediate for the synthesis of calcium (R)-
pantothenate and (R)-panthenol used as food additives. The commercial production of (R) …
pantothenate and (R)-panthenol used as food additives. The commercial production of (R) …
Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature
X Pei, Q Wang, L Meng, J Li, Z Yang, X Yin… - Journal of …, 2015 - Elsevier
Nitrile hydratase (NHase) is an important industrial enzyme that biosynthesizes high-value
amides. However, most of NHases expressed in Escherichia coli easily aggregate to …
amides. However, most of NHases expressed in Escherichia coli easily aggregate to …
Evidence for the participation of an extra α-helix at β-subunit surface in the thermal stability of Co-type nitrile hydratase
X Pei, J Wang, Y Wu, X Zhen, M Tang, Q Wang… - Applied microbiology …, 2018 - Springer
Nitrile hydratase (NHase) has attracted considerable attention since it can efficiently
catalyze the hydration of nitriles to valuable amides. However, the poor stability of NHase is …
catalyze the hydration of nitriles to valuable amides. However, the poor stability of NHase is …