Small molecules, such as solvent, substrate, and cofactor molecules, are key players in
enzyme catalysis. Computational methods are powerful tools for exploring the dynamics and …

Limosilactobacillus reuteri 121 4, 6-α-glucanotransferase GtfB (Lr 121 GtfB), belonging to
glycoside hydrolase family 70 (GH70), synthesizes linear isomalto/malto polysaccharides …

The desolvation of ionizable residues in the active sites of enzymes and the subsequent
effects on catalysis and thermostability have been studied in model systems, yet little about …

Class Ic ribonucleotide reductase (RNR) from Chlamydia trachomatis (C. tm.) lacks the
tyrosyl radical and uses a Mn (IV)− Fe (III) cluster for cysteinyl radical initiation in the large …

Phosphoenolpyruvate (PEP) carboxykinases catalyse the reversible formation of
oxaloacetate (OAA) and ATP (or GTP) from PEP, ADP (or GDP) and CO2. They are activated …

The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TlGK) relies on
the highly conserved motifs NXXE (ie Asn-Xaa-Xaa-Glu) and HXE (ie His-Xaa-Glu). Site …

Rising global temperatures require innovative measures to reduce atmospheric
concentrations of CO2. The most successful carbon capture technology on Earth is the …

The electrostatic potential plays a key role in many biological processes like determining the
affinity of a ligand to a given protein target, and they are responsible for the catalytic activity …

The Chlorobium tepidum ferritin (CtFtn) gene was synthesized and cloned into a pET3a
expression vector (Novagen). CtFtn was expressed in Escherichia coli and purified to …

Saccharomyces cerevisiae phosphoenolpyruvate (PEP) carboxykinase catalyzes the
reversible formation of oxaloacetate and adenosine triphosphate from PEP, adenosine …