Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …
to specific molecules with high affinity, and offer a number of advantages relative to …
Assays to estimate the binding affinity of aptamers
R Thevendran, M Citartan - Talanta, 2022 - Elsevier
Aptamers have become coming-of-age molecular recognition elements in both diagnostic
and therapeutic applications. Generated by SELEX, the 'quality control'of aptamers, which …
and therapeutic applications. Generated by SELEX, the 'quality control'of aptamers, which …
Pushing adenosine and ATP SELEX for DNA aptamers with nanomolar affinity
The classical DNA aptamer for adenosine and ATP has been the most used small molecule
binding aptamer for biosensing, imaging, and DNA nanotechnology. This sequence has …
binding aptamer for biosensing, imaging, and DNA nanotechnology. This sequence has …
Label-free and dye-free fluorescent sensing of tetracyclines using a capture-selected DNA aptamer
Y Zhao, S Ong, Y Chen, PJ Jimmy Huang… - Analytical …, 2022 - ACS Publications
Tetracyclines are a group of important antibiotics with a common four-ring scaffold. While
most tetracyclines are currently used only in animals, their leaching into the environment …
most tetracyclines are currently used only in animals, their leaching into the environment …
Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers
O Alkhamis, Y Xiao - Journal of the American Chemical Society, 2022 - ACS Publications
Aptamers are oligonucleotide receptors with great potential for sensing and therapeutic
applications. They are isolated from random libraries through an in vitro method termed …
applications. They are isolated from random libraries through an in vitro method termed …
Selection of aptamers for sensing caffeine and discrimination of its three single demethylated analogues
With the growing consumption of caffeine-containing beverages, detection of caffeine has
become an important biomedical, bioanalytical, and environmental topic. We herein isolated …
become an important biomedical, bioanalytical, and environmental topic. We herein isolated …
Cross-Binding of Four Adenosine/ATP Aptamers to Caffeine, Theophylline, and Other Methylxanthines
The classical DNA aptamer for adenosine and ATP was selected twice using ATP as the
target in 1995 and 2005, respectively. In 2022, this motif appeared four more times from …
target in 1995 and 2005, respectively. In 2022, this motif appeared four more times from …
A DNA aptamer for theophylline with ultrahigh selectivity reminiscent of the classic RNA aptamer
Since the report of the RNA aptamer for theophylline, theophylline has become a key
molecule in chemical biology for designing RNA switches and riboswitches. In addition …
molecule in chemical biology for designing RNA switches and riboswitches. In addition …
Sensing adenosine and ATP by aptamers and gold nanoparticles: opposite trends of color change from domination of target adsorption instead of aptamer binding
The 27 mer DNA aptamer for adenosine and adenosine 5′-triphosphate (ATP) is a popular
model system for designing biosensors. Various strategies have been reported for label-free …
model system for designing biosensors. Various strategies have been reported for label-free …
Using exonucleases for aptamer characterization, engineering, and sensing
O Alkhamis, J Canoura, PT Ly… - Accounts of Chemical …, 2023 - ACS Publications
Conspectus Aptamers are short, single-stranded nucleic acids that have been selected from
random libraries to bind specific molecules with high affinity via an in vitro method termed …
random libraries to bind specific molecules with high affinity via an in vitro method termed …