Automated immunoassays used to evaluate thyroid function are vulnerable to different types
of interference that can affect clinical decisions. This review provides a detailed overview of …

Objective: The objectives of this report are to review the mechanisms of biotin interference
with streptavidin/biotin-based immunoassays, identify automated immunoassay systems …

Importance Biotinylated antibodies and analogues, with their strong binding to streptavidin,
are used in many clinical laboratory tests. Excess biotin in blood due to supplemental biotin …

Background: High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS)
and is expected to be adopted by a large number of patients. Biotin therapy leads to …

Background Laboratory tests that use streptavidin–biotin binding mechanisms have the
potential to be affected by high circulating biotin concentrations, which would produce …

Background Shifts in consumer behavior and clinical practice have increased reports of
biotin interference in immunoassays that incorporate biotin–streptavidin linkages in their …

Dietary biotin intake does not typically result in blood biotin concentrations that exceed
interference thresholds for in vitro diagnostic tests. However, recent trends of high-dose …

Background Biotin interference is a significant problem to which at-risk laboratories must
now be attuned. We sought to systematically characterize the nature of this interference in …

Biotin (vitamin B7 or H) is an essential cofactor for mammalian carboxylase enzymes that
are involved in important metabolic pathways in humans. 1-3 It is acquired from food …

Background Biotin interference in streptavidin-based immunoassays causes widespread
analytical distortions that may lead to clinical confusion, inappropriate patient management …