CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …

Next-generation CRISPR-based diagnostic tools for human diseases

T Wang, Z Wang, L Bai, X Zhang, J Feng, C Qian… - TrAC Trends in …, 2023 - Elsevier
Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

A mountable toilet system for personalized health monitoring via the analysis of excreta

S Park, DD Won, BJ Lee, D Escobedo… - Nature biomedical …, 2020 - nature.com
Technologies for the longitudinal monitoring of a person's health are poorly integrated with
clinical workflows, and have rarely produced actionable biometric data for healthcare …

CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

Y Cao, J Wu, B Pang, H Zhang, XC Le - Chemical Communications, 2021 - pubs.rsc.org
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA
crosslinker from a molecular transducer, which facilitated the assembly of gold …

Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics

J Song, JW Hegge, MG Mauk, J Chen… - Nucleic acids …, 2020 - academic.oup.com
Detection of disease-associated, cell-free nucleic acids in body fluids enables early
diagnostics, genotyping and personalized therapy, but is challenged by the low …

Applications of CRISPR/Cas9 technology in the treatment of lung cancer

C Jiang, X Lin, Z Zhao - Trends in molecular medicine, 2019 - cell.com
Since its emergence, the application of CRISPR-associated nuclease 9 (Cas9) technology
in cancer research has accelerated studies to investigate many aspects of treatment …

Novel nucleic acid detection strategies based on CRISPR‐Cas systems: From construction to application

C Zhu, C Liu, X Qiu, S Xie, W Li… - Biotechnology and …, 2020 - Wiley Online Library
Beyond their widespread application as genome‐editing and regulatory tools, clustered
regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated (Cas) …

CRISPR-based enrichment strategies for targeted sequencing

Z Schultzhaus, Z Wang, D Stenger - Biotechnology advances, 2021 - Elsevier
The ability to easily produce or procure sequencing data has expanded to be within the
reach of most clinics and research laboratories, but the complexity of sequence analysis …

A universal CRISPR/Cas9-based electrochemiluminescence probe for sensitive and single-base-specific DNA detection

L Wu, T Zhou, R Huang - Sensors and Actuators B: Chemical, 2022 - Elsevier
Electrochemiluminescence (ECL) is a sensitive detection technology. Commercial ECL
analyzer democratizes ECL as a convenient tool for clinical immunoassay. Still, commercial …

Detection and diagnostic utilization of cellular and cell-free tumor DNA

JC Dudley, M Diehn - Annual Review of Pathology: Mechanisms …, 2021 - annualreviews.org
Because cancer is caused by an accumulation of genetic mutations, mutant DNA released
by tumors can be used as a highly specific biomarker for cancer. Although this principle was …