Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels
JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …
development of new microscopes, fluorescent labels and analysis techniques has pushed …
Fluorescent probes for organelle-targeted bioactive species imaging
P Gao, W Pan, N Li, B Tang - Chemical science, 2019 - pubs.rsc.org
Bioactive species, including reactive oxygen species (ROS, including O2˙−, H2O2, HOCl,
1O2,˙ OH, HOBr, etc.), reactive nitrogen species (RNS, including ONOO−, NO, NO2, HNO …
1O2,˙ OH, HOBr, etc.), reactive nitrogen species (RNS, including ONOO−, NO, NO2, HNO …
General design strategy to precisely control the emission of fluorophores via a twisted intramolecular charge transfer (TICT) process
K Hanaoka, S Iwaki, K Yagi, T Myochin… - Journal of the …, 2022 - ACS Publications
Fluorogenic probes for bioimaging have become essential tools for life science and
medicine, and the key to their development is a precise understanding of the mechanisms …
medicine, and the key to their development is a precise understanding of the mechanisms …
STED super-resolved microscopy
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …
Small-molecule fluorescent probes for live-cell super-resolution microscopy
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …
cellular structures at the nanometer scale. Employing these techniques in living cells has …
Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges
MK Goshisht, N Tripathi, GK Patra, M Chaskar - Chemical Science, 2023 - pubs.rsc.org
Biological species, including reactive oxygen species (ROS), reactive sulfur species (RSS),
reactive nitrogen species (RNS), F−, Pd2+, Cu2+, Hg2+, and others, are crucial for the …
reactive nitrogen species (RNS), F−, Pd2+, Cu2+, Hg2+, and others, are crucial for the …
Discerning the chemistry in individual organelles with small‐molecule fluorescent probes
Principle has it that even the most advanced super‐resolution microscope would be futile in
providing biological insight into subcellular matrices without well‐designed fluorescent …
providing biological insight into subcellular matrices without well‐designed fluorescent …
A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …
Ki-67 acts as a biological surfactant to disperse mitotic chromosomes
S Cuylen, C Blaukopf, AZ Politi, T Müller-Reichert… - Nature, 2016 - nature.com
Eukaryotic genomes are partitioned into chromosomes that form compact and spatially well-
separated mechanical bodies during mitosis,,. This enables chromosomes to move …
separated mechanical bodies during mitosis,,. This enables chromosomes to move …
Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes
We demonstrate residual channel attention networks (RCAN) for the restoration and
enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data …
enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data …