Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Fluorescent probes for organelle-targeted bioactive species imaging

P Gao, W Pan, N Li, B Tang - Chemical science, 2019 - pubs.rsc.org
Bioactive species, including reactive oxygen species (ROS, including O2˙−, H2O2, HOCl,
1O2,˙ OH, HOBr, etc.), reactive nitrogen species (RNS, including ONOO−, NO, NO2, HNO …

General design strategy to precisely control the emission of fluorophores via a twisted intramolecular charge transfer (TICT) process

K Hanaoka, S Iwaki, K Yagi, T Myochin… - Journal of the …, 2022 - ACS Publications
Fluorogenic probes for bioimaging have become essential tools for life science and
medicine, and the key to their development is a precise understanding of the mechanisms …

STED super-resolved microscopy

G Vicidomini, P Bianchini, A Diaspro - Nature methods, 2018 - nature.com
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …

Small-molecule fluorescent probes for live-cell super-resolution microscopy

L Wang, MS Frei, A Salim… - Journal of the American …, 2018 - ACS Publications
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges

MK Goshisht, N Tripathi, GK Patra, M Chaskar - Chemical Science, 2023 - pubs.rsc.org
Biological species, including reactive oxygen species (ROS), reactive sulfur species (RSS),
reactive nitrogen species (RNS), F−, Pd2+, Cu2+, Hg2+, and others, are crucial for the …

Discerning the chemistry in individual organelles with small‐molecule fluorescent probes

W Xu, Z Zeng, JH Jiang, YT Chang… - Angewandte Chemie …, 2016 - Wiley Online Library
Principle has it that even the most advanced super‐resolution microscope would be futile in
providing biological insight into subcellular matrices without well‐designed fluorescent …

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

L Wang, M Tran, E D'Este, J Roberti, B Koch, L Xue… - Nature Chemistry, 2020 - nature.com
Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …

Ki-67 acts as a biological surfactant to disperse mitotic chromosomes

S Cuylen, C Blaukopf, AZ Politi, T Müller-Reichert… - Nature, 2016 - nature.com
Eukaryotic genomes are partitioned into chromosomes that form compact and spatially well-
separated mechanical bodies during mitosis,,. This enables chromosomes to move …

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes

J Chen, H Sasaki, H Lai, Y Su, J Liu, Y Wu… - Nature …, 2021 - nature.com
We demonstrate residual channel attention networks (RCAN) for the restoration and
enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data …