Initiation of mRNA translation is a major checkpoint for regulating level and fidelity of protein
synthesis. Being rate limiting in protein synthesis, translation initiation also represents the …

After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …

During translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-
tRNAifMet) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit …

In bacteria, the translation of genetic information can begin through at least three different
mechanisms: canonical or Shine-Dalgarno-led initiation, readthrough or 70S scanning …

Over the last few years, the technical breakthrough of single-particle cryo electron
microscopy (cryo-EM) thanks to the recent developments of direct electron detectors and …

Abstract e IF 5 B is a eukaryal translational GTP ase that catalyzes ribosomal subunit joining
to form elongation‐competent ribosomes. Despite its central role in protein synthesis, the …

Translation initiation factor 2 (IF2) promotes 30S initiation complex (IC) formation and 50S
subunit joining, which produces the 70S IC. The architecture of full-length IF2, determined by …

Initiation factor (IF) 2 controls the fidelity of translation initiation by selectively increasing the
rate of 50S ribosomal subunit joining to 30S initiation complexes (ICs) that carry an N-formyl …

Selection of correct aminoacyl (aa)-tRNA at the ribosomal A site is fundamental to
maintaining translational fidelity. Aa-tRNA selection is a multistep process facilitated by the …

Thermophilic proteins and enzymes are attractive for use in industrial applications due to
their resistance against heat and denaturants. Here, we report on a thermophilic protein that …