DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
CRISPR-Cas tools and their application in genetic engineering of human stem cells and organoids
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
[HTML][HTML] Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA
O Dmytrenko, GC Neumann, T Hallmark, DJ Keiser… - Nature, 2023 - nature.com
Bacterial abortive-infection systems limit the spread of foreign invaders by shutting down or
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …
Versatile detection with CRISPR/Cas system from applications to challenges
H Wu, X Chen, M Zhang, X Wang, Y Chen… - TrAC Trends in …, 2021 - Elsevier
CRISPR/Cas system is now playing an important role in biosensing applications, especially
when the trans cleavage activity of several Cas effectors is discovered. Recently …
when the trans cleavage activity of several Cas effectors is discovered. Recently …
In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor
Y Li, Y Wu, R Xu, J Guo, F Quan, Y Zhang… - Nature …, 2023 - nature.com
Mutations in mitochondrial DNA (mtDNA) play critical roles in many human diseases. In vivo
visualization of cells bearing mtDNA mutations is important for resolving the complexity of …
visualization of cells bearing mtDNA mutations is important for resolving the complexity of …
CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement
A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …
from today. To meet the demands of growing, geographically and socio-economically …
Quantum dot-based molecular beacons for quantitative detection of nucleic acids with CRISPR/Cas (N) nucleases
CM Green, J Spangler, K Susumu, DA Stenger… - ACS …, 2022 - ACS Publications
Strategies utilizing the CRISPR/Cas nucleases Cas13 and Cas12 have shown great
promise in the development of highly sensitive and rapid diagnostic assays for the detection …
promise in the development of highly sensitive and rapid diagnostic assays for the detection …
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics
The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins.
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …