The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

At each cell division in humans, 30,000–50,000 DNA replication origins are activated, and it
remains unclear how they are selected and recognized by replication factors. DNA …

The origin recognition complex (ORC) is essential for initiation of eukaryotic chromosome
replication as it loads the replicative helicase—the minichromosome maintenance (MCM) …

Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …

The origin recognition complex (ORC) specifies replication origin location. The
Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) …

Eukaryotic DNA replication is regulated to ensure all chromosomes replicate once and only
once per cell cycle. Replication begins at many origins scattered along each chromosome …

The size of a eukaryotic genome presents a unique challenge to the cell: package and
organize the DNA to fit within the confines of the nucleus while at the same time ensuring …

We describe a multiplex genome engineering technology in Saccharomyces cerevisiae
based on annealing synthetic oligonucleotides at the lagging strand of DNA replication. The …

DNA replication in all cells begins with the melting of base pairs at the duplex origin to allow
access to single-stranded DNA templates which are replicated by DNA polymerases. In …

Many fundamental aspects of DNA replication, such as the exact locations where DNA
synthesis is initiated and terminated, how frequently origins are used, and how fork …