According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

CRISPR-Cas9 genome editing has enabled advanced T cell therapies, but occasional loss
of the targeted chromosome remains a safety concern. To investigate whether Cas9-induced …

Purpose: Using gene-disrupted allogeneic T cells as universal effector cells provides an
alternative and potentially improves current chimeric antigen receptor (CAR) T-cell therapy …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated
enzyme (Cas) is a naturally occurring genome editing tool adopted from the prokaryotic …

Despite the fact that great efforts have been made in the prevention and therapy of HIV-1
infection, HIV-1/AIDS remains a major threat to global human health. Highly active …

Interest in CRISPR technology, an instrumental component of prokaryotic adaptive immunity
which enables prokaryotes to detect any foreign DNA and then destroy it, has gained …