Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescent analogs of biomolecular building blocks: design, properties, and applications

RW Sinkeldam, NJ Greco, Y Tor - Chemical reviews, 2010 - ACS Publications
Fluorescence spectroscopy, one of the most informative and sensitive analytical techniques,
has played and continues to play key roles in modern research. Indeed, unraveling the inner …

Surface functionalization of nanoparticles with polyethylene glycol: effects on protein adsorption and cellular uptake

B Pelaz, P del Pino, P Maffre, R Hartmann, M Gallego… - ACS …, 2015 - ACS Publications
Here we have investigated the effect of enshrouding polymer-coated nanoparticles (NPs)
with polyethylene glycol (PEG) on the adsorption of proteins and uptake by cultured cells …

Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients

R Pal, TM Lwin, M Krishnamoorthy… - Nature Biomedical …, 2023 - nature.com
The surgical resection of solid tumours can be enhanced by fluorescence-guided imaging.
However, variable tumour uptake and incomplete clearance of fluorescent dyes reduces the …

Tunable heptamethine–azo dye conjugate as an NIR fluorescent probe for the selective detection of mitochondrial glutathione over cysteine and homocysteine

SY Lim, KH Hong, DI Kim, H Kwon… - Journal of the American …, 2014 - ACS Publications
Although a lot of mitochondria-targeting biothiol probes have been developed and applied
to cellular imaging through thiol-induced disulfide cleavage or Michael addition reactions …

Shortwave-infrared (SWIR) emitters for biological imaging: a review of challenges and opportunities

E Thimsen, B Sadtler, MY Berezin - Nanophotonics, 2017 - degruyter.com
Shortwave infrared radiation (SWIR) is the portion of the electromagnetic spectrum from
approximately 900 nm to 2500 nm. Recent advances in imaging systems have expanded …

Monitoring protein interactions and dynamics with solvatochromic fluorophores

GS Loving, M Sainlos, B Imperiali - Trends in biotechnology, 2010 - cell.com
Solvatochromic fluorophores possess emission properties that are sensitive to the nature of
the local microenvironment. These dyes have been exploited in applications ranging from …

Fluorous soluble cyanine dyes for visualizing perfluorocarbons in living systems

I Lim, A Vian, HL van de Wouw, RA Day… - Journal of the …, 2020 - ACS Publications
The bioorthogonal nature of perfluorocarbons provides a unique platform for introducing
dynamic nano-and microdroplets into cells and organisms. To monitor the localization and …

Noninvasive photoacoustic and fluorescence sentinel lymph node identification using dye-loaded perfluorocarbon nanoparticles

WJ Akers, C Kim, M Berezin, K Guo, R Fuhrhop… - ACS …, 2011 - ACS Publications
The contrast mechanisms used for photoacoustic tomography (PAT) and fluorescence
imaging differ in subtle, but significant, ways. The design of contrast agents for each or both …

Three-dimensional surface profile intensity correction for spatially modulated imaging

S Gioux, A Mazhar, DJ Cuccia… - … of biomedical optics, 2009 - spiedigitallibrary.org
We describe a noncontact profile correction technique for quantitative, wide-field optical
measurement of tissue absorption (μ a) and reduced scattering (μ s′) coefficients, based on …