Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

[HTML][HTML] Enzyme immobilization strategies for the design of robust and efficient biocatalysts

JM Guisan, G Fernandez-Lorente… - Current Opinion in …, 2022 - Elsevier
Different strategies for the preparation of efficient and robust immobilized biocatalysts are
here reviewed. Different physico-chemical approaches are discussed. i.-The stabilization of …

Effect of atmospheric cold plasma on structure, interfacial and emulsifying properties of Grass pea (Lathyrus sativus L.) protein isolate

HM Mehr, A Koocheki - Food Hydrocolloids, 2020 - Elsevier
Abstract Structure and emulsifying properties of Grass pea (Lathyrus sativus) protein isolate
(GPPI) were investigated as function of cold plasma treatment at two voltages (9.4 and 18.6 …

Chemical modification of enzymes to improve biocatalytic performance

P Giri, AD Pagar, MD Patil, H Yun - Biotechnology Advances, 2021 - Elsevier
Improvement in intrinsic enzymatic features is in many instances a prerequisite for the
scalable applicability of many industrially important biocatalysts. To this end, various …

Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents

X Wang, Y Sheng, H Cui, J Qiao, Y Song… - Angewandte …, 2024 - Wiley Online Library
Deep eutectic solvents (DESs), heralded for their synthesis simplicity, economic viability,
and reduced volatility and flammability, have found increasing application in biocatalysis …

Lecitase ultra: A phospholipase with great potential in biocatalysis

JJ Virgen-Ortiz, JCS dos Santos, C Ortiz… - Molecular …, 2019 - Elsevier
Lecitase Ultra is a chimera produced by the fusion of the genes of the lipase from
Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum. The …

Physicochemical properties of Grass pea (Lathyrus sativus L.) protein nanoparticles fabricated by cold atmospheric-pressure plasma

HM Mehr, A Koocheki - Food Hydrocolloids, 2021 - Elsevier
In the present study, the potential of cold atmospheric-pressure plasma (CAPP) for
fabrication of Grass pea protein isolate nanoparticles (GPPINPs) was investigated. The size …

Efficient production of a novel alkaline cold-active phospholipase C from Aspergillus oryzae by molecular chaperon co-expression for crude oil degumming

L Wang, T Hu, Z Jiang, Q Yan, S Yang - Food chemistry, 2021 - Elsevier
A novel alkaline cold-active phospholipase C (PLC) gene (AoPC) from Aspergillus oryzae
was cloned. AoPC exhibited the highest sequence similarity of 32.5% with that of a PLC from …

Progress & prospect of enzyme-mediated structured phospholipids preparation

Y Li, L Dai, D Liu, W Du - Catalysts, 2022 - mdpi.com
In recent years, structured phospholipids (SPLs), which are modified phospholipids (PLs),
have attracted more attention due to their great potential for application in the field of …

Rational engineering of phospholipase C from Bacillus cereus HSL3 for simultaneous thermostability and activity improvement

Y Zhang, P Dai, R Liu, W Liu, A Xiao, J Li, G Li… - Journal of …, 2022 - Elsevier
As an essential enzyme for phospholipid degradation, phospholipase C (PLC) has been
used for enzymatic degumming of vegetable oils and production of valuable phospholipid …