BACKGROUND Genes correspond to single transcription units, starting from the promoter
and ending at the terminator. Terminating gene transcription is directly coupled to mRNA …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …

Transcription is a highly dynamic process. Consequently, we have developed native
elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which …

Much of the mammalian genome is transcribed, generating long non-coding RNAs
(lncRNAs) that can undergo post-transcriptional surveillance whereby only a subset of the …

Production of mRNA depends critically on the rate of RNA polymerase II (Pol II) elongation.
To dissect Pol II dynamics in mouse ES cells, we inhibited Pol II transcription at either …

Dissecting the regulatory mechanisms controlling mammalian transcripts from production to
degradation requires quantitative measurements of mRNA flow across the cell. We …

Recent improvements in experimental and computational techniques that are used to study
the transcriptome have enabled an unprecedented view of RNA processing, revealing many …

Background A critical step in uncovering rules of RNA processing is to study the in vivo
regulatory networks of RNA binding proteins (RBPs). Crosslinking and immunoprecipitation …

Numerous long intervening noncoding RNAs (lincRNAs) are generated from the mammalian
genome by RNA polymerase II (Pol II) transcription. Although multiple functions have been …

Pre-mRNA maturation frequently occurs at the same time and place as transcription by RNA
polymerase II. The co-transcriptionality of mRNA processing has permitted the evolution of …