Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Structured illumination microscopy (SIM) has become the standard for next-generation wide-
field microscopy, offering ultrahigh imaging speed, superresolution, a large field-of-view …

A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is
the maximum photon flux that can be collected. To further increase the effective resolution …

SARS-CoV-2 proteases Mpro and PLpro are promising targets for antiviral drug
development. In this study, we present an antiviral screening strategy involving a novel in …

Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a
complete understanding of their intricate organization requires the nanometre-level, three …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Selective autophagy is the lysosomal degradation of specific intracellular components
sequestered into autophagosomes, late endosomes, or lysosomes through the activity of …

We present ilastik, an easy-to-use interactive tool that brings machine-learning-based (bio)
image analysis to end users without substantial computational expertise. It contains pre …

Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized
interactions capable of orchestrating complex cellular functions. Visualizing these …