Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
The new frontier of genome engineering with CRISPR-Cas9
JA Doudna, E Charpentier - Science, 2014 - science.org
Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …
biology ever since the discovery of the DNA double helix. But introducing site-specific …
CRISPR/Cas9 in genome editing and beyond
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
Genetically engineered mouse models in oncology research and cancer medicine
K Kersten, KE de Visser, MH van Miltenburg… - EMBO molecular …, 2017 - embopress.org
Genetically engineered mouse models (GEMM s) have contributed significantly to the field of
cancer research. In contrast to cancer cell inoculation models, GEMM s develop de novo …
cancer research. In contrast to cancer cell inoculation models, GEMM s develop de novo …
CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons
Standard CRISPR-mediated gene disruption strategies rely on Cas9-induced DNA double-
strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently …
strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently …
Genome-editing technologies: principles and applications
Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system
Chromosomal rearrangements have a central role in the pathogenesis of human cancers
and often result in the expression of therapeutically actionable gene fusions. A recently …
and often result in the expression of therapeutically actionable gene fusions. A recently …
Expanding the biologist's toolkit with CRISPR-Cas9
SH Sternberg, JA Doudna - Molecular cell, 2015 - cell.com
Few discoveries transform a discipline overnight, but biologists today can manipulate cells in
ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity …
ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity …
Applications of the CRISPR–Cas9 system in cancer biology
FJ Sánchez-Rivera, T Jacks - Nature reviews cancer, 2015 - nature.com
The prokaryotic type II CRISPR–Cas9 (clustered regularly interspaced short palindromic
repeats–CRISPR-associated 9) system is rapidly revolutionizing the field of genetic …
repeats–CRISPR-associated 9) system is rapidly revolutionizing the field of genetic …