Challenges and opportunities for the next generation of cardiovascular tissue engineering

S Cho, DE Discher, KW Leong, G Vunjak-Novakovic… - Nature …, 2022 - nature.com
Engineered cardiac tissues derived from human induced pluripotent stem cells offer unique
opportunities for patient-specific disease modeling, drug discovery and cardiac repair. Since …

Cardiomyocyte maturation: new phase in development

Y Guo, WT Pu - Circulation research, 2020 - Am Heart Assoc
Maturation is the last phase of heart development that prepares the organ for strong,
efficient, and persistent pumping throughout the mammal's lifespan. This process is …

Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

JU Lind, TA Busbee, AD Valentine, FS Pasqualini… - Nature materials, 2017 - nature.com
Biomedical research has relied on animal studies and conventional cell cultures for
decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that …

Functional screening in human cardiac organoids reveals a metabolic mechanism for cardiomyocyte cell cycle arrest

RJ Mills, DM Titmarsh, X Koenig… - Proceedings of the …, 2017 - National Acad Sciences
The mammalian heart undergoes maturation during postnatal life to meet the increased
functional requirements of an adult. However, the key drivers of this process remain poorly …

Microfluidic organs-on-chips

SN Bhatia, DE Ingber - Nature biotechnology, 2014 - nature.com
An organ-on-a-chip is a microfluidic cell culture device created with microchip manufacturing
methods that contains continuously perfused chambers inhabited by living cells arranged to …

Biodegradable scaffold with built-in vasculature for organ-on-a-chip engineering and direct surgical anastomosis

B Zhang, M Montgomery, MD Chamberlain, S Ogawa… - Nature materials, 2016 - nature.com
We report the fabrication of a scaffold (hereafter referred to as AngioChip) that supports the
assembly of parenchymal cells on a mechanically tunable matrix surrounding a perfusable …

Measuring cell-generated forces: a guide to the available tools

WJ Polacheck, CS Chen - Nature methods, 2016 - nature.com
Forces generated by cells are critical regulators of cell adhesion, signaling, and function,
and they are also essential drivers in the morphogenetic events of development. Over the …

Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness

AJS Ribeiro, YS Ang, JD Fu, RN Rivas… - Proceedings of the …, 2015 - National Acad Sciences
Single cardiomyocytes contain myofibrils that harbor the sarcomere-based contractile
machinery of the myocardium. Cardiomyocytes differentiated from human pluripotent stem …

Organs-on-a-chip: a union of tissue engineering and microfabrication

Y Zhao, EY Wang, FBL Lai, K Cheung, M Radisic - Trends in biotechnology, 2023 - cell.com
We review the emergence of the new field of organ-on-a-chip (OOAC) engineering, from the
parent fields of tissue engineering and microfluidics. We place into perspective the tools and …

Beating heart on a chip: a novel microfluidic platform to generate functional 3D cardiac microtissues

A Marsano, C Conficconi, M Lemme, P Occhetta… - Lab on a Chip, 2016 - pubs.rsc.org
In the past few years, microfluidic-based technology has developed microscale models
recapitulating key physical and biological cues typical of the native myocardium. However …