Phage display derived monoclonal antibodies: from bench to bedside

MA Alfaleh, HO Alsaab, AB Mahmoud… - Frontiers in …, 2020 - frontiersin.org
Monoclonal antibodies (mAbs) have become one of the most important classes of
biopharmaceutical products, and they continue to dominate the universe of …

Phage display-derived human antibodies in clinical development and therapy

A Frenzel, T Schirrmann, M Hust - MAbs, 2016 - Taylor & Francis
Over the last 3 decades, monoclonal antibodies have become the most important class of
therapeutic biologicals on the market. Development of therapeutic antibodies was …

IgLM: Infilling language modeling for antibody sequence design

RW Shuai, JA Ruffolo, JJ Gray - Cell Systems, 2023 - cell.com
Discovery and optimization of monoclonal antibodies for therapeutic applications relies on
large sequence libraries but is hindered by developability issues such as low solubility, high …

scFv antibody: principles and clinical application

ZA Ahmad, SK Yeap, AM Ali, WY Ho… - Journal of …, 2012 - Wiley Online Library
To date, generation of single‐chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen‐binding fragment in bacterial …

RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking

EV Dolgosheina, SCY Jeng… - ACS chemical …, 2014 - ACS Publications
Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA
molecules in real time. To address this problem and to allow the purification of fluorescently …

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

JJ Agresti, E Antipov, AR Abate, K Ahn… - Proceedings of the …, 2010 - National Acad Sciences
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving
ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively …

Yeast surface display for screening combinatorial polypeptide libraries

ET Boder, KD Wittrup - Nature biotechnology, 1997 - nature.com
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and
secreted proteins (eg, antibodies, receptors, cytokines) that require endoplasmic reticulum …

Immunogenicity and humanization of single‐domain antibodies

MA Rossotti, K Bélanger, KA Henry… - The FEBS Journal, 2022 - Wiley Online Library
Single‐domain antibodies (sdAbs), the autonomous variable domains of camelid and shark
heavy‐chain antibodies, have many desirable properties as components of biologic drugs …

Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies

M Arbabi Ghahroudi, A Desmyter, L Wyns… - FEBS …, 1997 - Wiley Online Library
Functional heavy‐chain γ‐immunoglobulins lacking light chains occur naturally in
Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire …

Selecting and screening recombinant antibody libraries

HR Hoogenboom - Nature biotechnology, 2005 - nature.com
During the past decade several display methods and other library screening techniques
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …