Phage display derived monoclonal antibodies: from bench to bedside
MA Alfaleh, HO Alsaab, AB Mahmoud… - Frontiers in …, 2020 - frontiersin.org
Monoclonal antibodies (mAbs) have become one of the most important classes of
biopharmaceutical products, and they continue to dominate the universe of …
biopharmaceutical products, and they continue to dominate the universe of …
Phage display-derived human antibodies in clinical development and therapy
A Frenzel, T Schirrmann, M Hust - MAbs, 2016 - Taylor & Francis
Over the last 3 decades, monoclonal antibodies have become the most important class of
therapeutic biologicals on the market. Development of therapeutic antibodies was …
therapeutic biologicals on the market. Development of therapeutic antibodies was …
IgLM: Infilling language modeling for antibody sequence design
Discovery and optimization of monoclonal antibodies for therapeutic applications relies on
large sequence libraries but is hindered by developability issues such as low solubility, high …
large sequence libraries but is hindered by developability issues such as low solubility, high …
scFv antibody: principles and clinical application
To date, generation of single‐chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen‐binding fragment in bacterial …
technique used to produce a completely functional antigen‐binding fragment in bacterial …
RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking
EV Dolgosheina, SCY Jeng… - ACS chemical …, 2014 - ACS Publications
Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA
molecules in real time. To address this problem and to allow the purification of fluorescently …
molecules in real time. To address this problem and to allow the purification of fluorescently …
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
JJ Agresti, E Antipov, AR Abate, K Ahn… - Proceedings of the …, 2010 - National Acad Sciences
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving
ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively …
ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively …
Yeast surface display for screening combinatorial polypeptide libraries
ET Boder, KD Wittrup - Nature biotechnology, 1997 - nature.com
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and
secreted proteins (eg, antibodies, receptors, cytokines) that require endoplasmic reticulum …
secreted proteins (eg, antibodies, receptors, cytokines) that require endoplasmic reticulum …
Immunogenicity and humanization of single‐domain antibodies
MA Rossotti, K Bélanger, KA Henry… - The FEBS Journal, 2022 - Wiley Online Library
Single‐domain antibodies (sdAbs), the autonomous variable domains of camelid and shark
heavy‐chain antibodies, have many desirable properties as components of biologic drugs …
heavy‐chain antibodies, have many desirable properties as components of biologic drugs …
Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies
M Arbabi Ghahroudi, A Desmyter, L Wyns… - FEBS …, 1997 - Wiley Online Library
Functional heavy‐chain γ‐immunoglobulins lacking light chains occur naturally in
Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire …
Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire …
Selecting and screening recombinant antibody libraries
HR Hoogenboom - Nature biotechnology, 2005 - nature.com
During the past decade several display methods and other library screening techniques
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …