The first microbial colonizers of the human gut: composition, activities, and health implications of the infant gut microbiota

C Milani, S Duranti, F Bottacini, E Casey… - Microbiology and …, 2017 - Am Soc Microbiol
The human gut microbiota is engaged in multiple interactions affecting host health during
the host's entire life span. Microbes colonize the neonatal gut immediately following birth …

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

RM Bowers, NC Kyrpides, R Stepanauskas… - Nature …, 2017 - nature.com
We present two standards developed by the Genomic Standards Consortium (GSC) for
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …

Recovery of nearly 8,000 metagenome-assembled genomes substantially expands the tree of life

DH Parks, C Rinke, M Chuvochina, PA Chaumeil… - Nature …, 2017 - nature.com
Challenges in cultivating microorganisms have limited the phylogenetic diversity of currently
available microbial genomes. This is being addressed by advances in sequencing …

Microbial abundance, activity and population genomic profiling with mOTUs2

A Milanese, DR Mende, L Paoli, G Salazar… - Nature …, 2019 - nature.com
Metagenomic sequencing has greatly improved our ability to profile the composition of
environmental and host-associated microbial communities. However, the dependency of …

Isothermal amplification of nucleic acids

Y Zhao, F Chen, Q Li, L Wang, C Fan - Chemical reviews, 2015 - ACS Publications
Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …

Prokaryotic taxonomy and nomenclature in the age of big sequence data

P Hugenholtz, M Chuvochina, A Oren… - the ISME …, 2021 - academic.oup.com
The classification of life forms into a hierarchical system (taxonomy) and the application of
names to this hierarchy (nomenclature) is at a turning point in microbiology. The …

Single‐cell analysis using droplet microfluidics

K Matuła, F Rivello, WTS Huck - Advanced Biosystems, 2020 - Wiley Online Library
Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …

Unusual biology across a group comprising more than 15% of domain Bacteria

CT Brown, LA Hug, BC Thomas, I Sharon, CJ Castelle… - Nature, 2015 - nature.com
A prominent feature of the bacterial domain is a radiation of major lineages that are defined
as candidate phyla because they lack isolated representatives. Bacteria from these phyla …

Ecology and exploration of the rare biosphere

MDJ Lynch, JD Neufeld - Nature Reviews Microbiology, 2015 - nature.com
The profound influence of microorganisms on human life and global biogeochemical cycles
underlines the value of studying the biogeography of microorganisms, exploring microbial …

Single-cell RNA-seq: advances and future challenges

AE Saliba, AJ Westermann, SA Gorski… - Nucleic acids …, 2014 - academic.oup.com
Phenotypically identical cells can dramatically vary with respect to behavior during their
lifespan and this variation is reflected in their molecular composition such as the …