Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

Automation and miniaturization are key issues of high-throughput research projects in the
post-genomic era. The implementation of robotics and parallelization has enabled …

The gram‐negative bacterium Escherichia coli offers a mean for rapid, high yield, and
economical production of recombinant proteins. However, high‐level production of …

Tobacco etch virus NIa proteinase (TEV protease) is an important tool for the removal of
fusion tags from recombinant proteins. Production of TEV protease in Escherichia coli has …

Thalidomide possesses two optical isomers which have been reported to exhibit different
pharmacological and toxicological activities. However, the precise mechanism by which the …

Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid.
Deficiencies in this enzyme have been linked to autoimmune diseases and neurological …

The auto‐induction method of protein expression in E. coli is based on diauxic growth
resulting from dynamic function of lac operon regulatory elements (lacO and LacI) in …

A thorough characterisation of the genetics, physiology and metabolism of Escherichia coli
has led to the availability of a large number of strains and vectors suitable for recombinant …

Eukaryotic proteasome consists of a core particle (CP), which degrades unfolded protein,
and a regulatory particle (RP), which is responsible for recognition, ATP-dependent …

Production of milligram quantities of numerous proteins for structural and functional studies
requires an efficient purification pipeline. We found that the dual tag, his6-tag–maltose …