CRISPR technology: A decade of genome editing is only the beginning

JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

Understanding 3D genome organization by multidisciplinary methods

I Jerkovic, G Cavalli - Nature Reviews Molecular Cell Biology, 2021 - nature.com
Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing

X Xu, A Chemparathy, L Zeng, HR Kempton, S Shang… - Molecular cell, 2021 - cell.com
Compact and versatile CRISPR-Cas systems will enable genome engineering applications
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …

CRISPR technologies for precise epigenome editing

M Nakamura, Y Gao, AA Dominguez, LS Qi - Nature Cell Biology, 2021 - nature.com
The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

[HTML][HTML] Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza

TR Abbott, G Dhamdhere, Y Liu, X Lin, L Goudy… - Cell, 2020 - cell.com
Summary The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-
2 virus, has highlighted the need for antiviral approaches that can target emerging viruses …

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …

Massively parallel Cas13 screens reveal principles for guide RNA design

HH Wessels, A Méndez-Mancilla, X Guo, M Legut… - Nature …, 2020 - nature.com
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable
specific and robust target gene knockdown without altering the genome. To define rules for …

MyoD is a 3D genome structure organizer for muscle cell identity

R Wang, F Chen, Q Chen, X Wan, M Shi… - Nature …, 2022 - nature.com
The genome exists as an organized, three-dimensional (3D) dynamic architecture, and each
cell type has a unique 3D genome organization that determines its cell identity. An …

DM3Loc: multi-label mRNA subcellular localization prediction and analysis based on multi-head self-attention mechanism

D Wang, Z Zhang, Y Jiang, Z Mao, D Wang… - Nucleic acids …, 2021 - academic.oup.com
Subcellular localization of messenger RNAs (mRNAs), as a prevalent mechanism, gives
precise and efficient control for the translation process. There is mounting evidence for the …

CRISPR mediated biosensing toward understanding cellular biology and point‐of‐care diagnosis

Y Dai, Y Wu, G Liu, JJ Gooding - … Chemie International Edition, 2020 - Wiley Online Library
Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities
to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases …