The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for
sequence-specific regulation of gene expression. This Review describes the development of …

Genome‐scale metabolic models (GEM s) are widely used to calculate metabolic
phenotypes. They rely on defining a set of constraints, the most common of which is that the …

Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …

Signals in many biological processes can be amplified by recruiting multiple copies of
regulatory proteins to a site of action. Harnessing this principle, we have developed a …

Direct or indirect exposure to inorganic arsenic (iAs) in the forms of AsIII (arsenite) and AsV
(arsenate) through consumption of As-contaminated food materials and drinking water leads …

Viruses are a common threat to cellular life, not the least to bacteria and archaea who
constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus …

The most widely used approach for defining gene function is to reduce or completely disrupt
its normal expression. For over a decade, RNAi has ruled the lab, offering a magic bullet to …

Optogenetic systems enable precise spatial and temporal control of cell behavior. We
engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription …