DNA methylation cancer biomarkers: translation to the clinic
Carcinogenesis is accompanied by widespread DNA methylation changes within the cell.
These changes are characterized by a globally hypomethylated genome with focal …
These changes are characterized by a globally hypomethylated genome with focal …
[PDF][PDF] Practical considerations for large-scale gut microbiome studies
D Vandeputte, RY Tito, R Vanleeuwen… - FEMS microbiology …, 2017 - academic.oup.com
First insights on the human gut microbiome have been gained from medium-sized, cross-
sectional studies. However, given the modest portion of explained variance of currently …
sectional studies. However, given the modest portion of explained variance of currently …
Caterpillars lack a resident gut microbiome
Many animals are inhabited by microbial symbionts that influence their hosts' development,
physiology, ecological interactions, and evolutionary diversification. However, firm evidence …
physiology, ecological interactions, and evolutionary diversification. However, firm evidence …
16S rRNA gene sequencing of mock microbial populations-impact of DNA extraction method, primer choice and sequencing platform
F Fouhy, AG Clooney, C Stanton, MJ Claesson… - BMC microbiology, 2016 - Springer
Background Next-generation sequencing platforms have revolutionised our ability to
investigate the microbiota composition of complex environments, frequently through 16S …
investigate the microbiota composition of complex environments, frequently through 16S …
The effects of freezing on faecal microbiota as determined using MiSeq sequencing and culture-based investigations
Background High-throughput sequencing has enabled detailed insights into complex
microbial environments, including the human gut microbiota. The accuracy of the …
microbial environments, including the human gut microbiota. The accuracy of the …
Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of
oral microbiota. However, it remains not clear whether and to what degree DNA extraction …
oral microbiota. However, it remains not clear whether and to what degree DNA extraction …
Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis
A Salonen, J Nikkilä, J Jalanka-Tuovinen… - Journal of …, 2010 - Elsevier
Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …
The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing
Introduction Determining bacterial community structure in fecal samples through DNA
sequencing is an important facet of intestinal health research. The impact of different …
sequencing is an important facet of intestinal health research. The impact of different …
Methodology challenges in studying human gut microbiota–effects of collection, storage, DNA extraction and next generation sequencing technologies
The information on microbiota composition in the human gastrointestinal tract predominantly
originates from the analyses of human faeces by application of next generation sequencing …
originates from the analyses of human faeces by application of next generation sequencing …
Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples
Storage conditions are considered to be a critical component of DNA-based microbial
community analysis methods. However, whether differences in short-term sample storage …
community analysis methods. However, whether differences in short-term sample storage …