Publisher Summary Escherichia coli is a universal host organism both for molecular cloning
of DNA and for a diverse set of assays involving clones genes. This chapter discusses the …

Site-specific recombinase systems (Cre-loxP, Flp-FRT, and φC31-att) are transforming both
forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be …

We have developed a simple and highly efficient method to disrupt chromosomal genes in
Escherichia coli in which PCR primers provide the homology to the targeted gene (s). In this …

An essential resource for all scientists researching cellular responses to DNA damage.•
Introduces important new material reflective of the major changes and developments that …

A straightforward way to engineer DNA in E. coli using homologous recombination is
described. The homologous recombination reaction uses RecE and RecT and is …

Invasion of the intestinal epithelium is thought to be an important step in the pathogenesis of
Salmonella infections. Using an in vitro system, we have isolated a genetic locus, inv, that …

A starvation-inducible DNA-binding protein was discovered as a result of the analysis of
proteins synthesized in 3-day-old cultures of Escherichia coli. This 19-kD protein …

Replacement of Escherichia coli's RecBCD function with phage λ's Red function generates a
strain whose chromosome recombines with short linear DNA fragments at a greatly elevated …

We have developed a new system of chromosomal mutagenesis in order to study the
functions of uncharacterized open reading frames (ORFs) in wild-type Escherichia coli …

Highly efficient phage-based Escherichia coli homologous recombination systems have
recently been developed that enable genomic DNA in bacterial artificial chromosomes to be …