CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

The historical reliance of biological research on the use of animal models has sometimes
made it challenging to address questions that are specific to the understanding of human …

Mutation-induced activation of WNT–β-catenin signalling is a frequent driver event in human
cancer. Sustained WNT–β-catenin pathway activation endows cancer cells with sustained …

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …

Tumor organoids have been proposed as a model system for precision medicine. The ability
of tumor organoids to retain characteristics of the original tumor makes them unique for …

A delicate equilibrium of WNT agonists and antagonists in the intestinal stem cell (ISC) niche
is critical to maintaining the ISC compartment, as it accommodates the rapid renewal of the …

For decades, insight into fundamental principles of human biology and disease has been
obtained primarily by experiments in animal models. While this has allowed researchers to …

Synthetic lethality (SL) provides a conceptual framework for tackling targets that are not
classically “druggable,” including loss-of-function mutations in tumor suppressor genes …

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …

Organoid technologies enable the creation of in vitro physiologic systems that model tissues
of origin more accurately than classical culture approaches. Seminal characteristics of these …