The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-
Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward …

Staphylococcus aureus is an important human pathogen, but studies of the organism have
suffered from the lack of a robust tool set for its genetic and genomic manipulation. Here we …

Previous studies have modified microbial genomes by introducing gene cassettes
containing selectable markers and homologous DNA fragments. However, this requires …

Ribonuclease HII (RNaseHII) is the principal enzyme that removes misincorporated
ribonucleoside monophosphates (rNMPs) from genomic DNA. Here, we present structural …

Bp7 is a T-even phage with a broad host range specific to Escherichia coli, including E. coli
K-12. The receptor binding protein (RBP) of bacteriophages plays an important role in the …

Diverse Lactobacillus strains are widely used as probiotic cultures in the dairy and dietary
supplement industries, and specific strains, such as Lactobacillus acidophilus NCFM, have …

Despite the advent of new techniques for genetic engineering of bacteria, allelic exchange
through homologous recombination remains an important tool for genetic analysis …

The sequence-specific RNA-binding protein CsrA is the central component of the conserved
global regulatory Csr system. In Escherichia coli, CsrA regulates many cellular processes …

Powered by ion transport across the cell membrane, conserved ion-powered rotary motors
(IRMs) drive bacterial motility by generating torque on the rotor of the bacterial flagellar …

A broad variety of biomolecules is industrially produced in bacteria and yeasts. These
microbial expression hosts can be optimized through genetic engineering using CRISPR …