Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines

AO Olivares, TA Baker, RT Sauer - Nature Reviews Microbiology, 2016 - nature.com
To maintain protein homeostasis, AAA+ proteolytic machines degrade damaged and
unneeded proteins in bacteria, archaea and eukaryotes. This process involves the ATP …

Spiraling in control: structures and mechanisms of the Hsp104 disaggregase

J Shorter, DR Southworth - Cold Spring Harbor …, 2019 - cshperspectives.cshlp.org
Hsp104 is a hexameric AAA+ ATPase and protein disaggregase found in yeast, which
couples ATP hydrolysis to the dissolution of diverse polypeptides trapped in toxic …

Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104

SN Gates, AL Yokom, JB Lin, ME Jackrel, AN Rizo… - Science, 2017 - science.org
Hsp100 polypeptide translocases are conserved members of the AAA+ family (adenosine
triphosphatases associated with diverse cellular activities) that maintain proteostasis by …

Processive extrusion of polypeptide loops by a Hsp100 disaggregase

MJ Avellaneda, KB Franke, V Sunderlikova, B Bukau… - Nature, 2020 - nature.com
The ability to reverse protein aggregation is vital to cells,. Hsp100 disaggregases such as
ClpB and Hsp104 are proposed to catalyse this reaction by translocating polypeptide loops …

Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase

AN Rizo, JB Lin, SN Gates, E Tse, SM Bart… - Nature …, 2019 - nature.com
Abstract Bacterial ClpB and yeast Hsp104 are homologous Hsp100 protein disaggregases
that serve critical functions in proteostasis by solubilizing protein aggregates. Two AAA+ …

A review: Molecular chaperone-mediated folding, unfolding and disaggregation of expressed recombinant proteins

K Fatima, F Naqvi, H Younas - Cell Biochemistry and Biophysics, 2021 - Springer
The advancements in biotechnology over time have led to an increase in the demand of
pure, soluble and functionally active proteins. Recombinant protein production has thus …

Mechanical protein unfolding and degradation

AO Olivares, TA Baker, RT Sauer - Annual review of physiology, 2018 - annualreviews.org
AAA+ proteolytic machines use energy from ATP hydrolysis to degrade damaged, misfolded,
or unneeded proteins. Protein degradation occurs within a barrel-shaped self …

Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation

AL Yokom, SN Gates, ME Jackrel, KL Mack… - Nature structural & …, 2016 - nature.com
Hsp104, a conserved AAA+ protein disaggregase, promotes survival during cellular stress.
Hsp104 remodels amyloids, thereby supporting prion propagation, and disassembles toxic …

Mechanistic and structural insights into the prion-disaggregase activity of Hsp104

EA Sweeny, J Shorter - Journal of molecular biology, 2016 - Elsevier
Hsp104 is a dynamic ring translocase and hexameric AAA+ protein found in yeast, which
couples ATP hydrolysis to disassembly and reactivation of proteins trapped in soluble …

AAA+ proteins: one motor, multiple ways to work

JB Lin, J Shorter, AL Lucius - Biochemical Society Transactions, 2022 - portlandpress.com
Numerous ATPases associated with diverse cellular activities (AAA+) proteins form
hexameric, ring-shaped complexes that function via ATPase-coupled translocation of …