The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

A nearly forgotten reaction discovered more than 60 years ago—the cycloaddition of a cyclic
alkyne and an organic azide, leading to an aromatic triazole—enjoys a remarkable …

Bioorthogonal chemistry has enabled the selective labeling and detection of biomolecules in
living systems. Bioorthogonal smart probes, which become fluorescent or deliver imaging or …

We describe a protocol for the rapid labeling of cell-surface proteins in living mammalian
cells using click chemistry. The labeling method is based on strain-promoted alkyne-azide …

A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-
functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of …

A series of D-π-A fluorescent styryl pyrene probes were synthesized through the Wittig
reaction. The introduction of an electron-withdrawing substituent on the phenyl ring creates …

Highlights•Multiple noncanonical amino acids (ncAAs) can be incorporated site-specifically
into target proteins.•Clickable ncAAs can be used to attach small dyes directly to the target …

The synthesis of a set of tetrazine‐bearing fluorogenic dyes suitable for intracellular labeling
of proteins in live cells is presented. The red excitability and emission properties ensure …

The synthesis, fluorogenic characterization, and labeling application of four tetrazine-
quenched cyanine probes with emission maxima in the red–far red range is reported …

This review ventures to summarize the latest developments in bioorthogonal fluorescent
imaging labels with a special focus on bioimaging applications. We briefly summarize the …