Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …

Cryo-electron microscopy (cryoEM) is becoming the preferred method for resolving protein
structures. Low signal-to-noise ratio (SNR) in cryoEM images reduces the confidence and …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Structural biology studies inside cells and tissues require methods to thin vitrified specimens
to electron transparency. Until now, focused ion beams based on gallium have been used …

Sarcomeres are force-generating and load-bearing devices of muscles. A precise molecular
picture of how sarcomeres are built underpins understanding their role in health and …

Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …

Macromolecular crowding has a profound impact on reaction rates and the physical
properties of the cell interior, but the mechanisms that regulate crowding are poorly …