Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives

H Østby, LD Hansen, SJ Horn… - Journal of Industrial …, 2020 - academic.oup.com
Efficient saccharification of lignocellulosic biomass requires concerted development of a
pretreatment method, an enzyme cocktail and an enzymatic process, all of which are …

Role and significance of lytic polysaccharide monooxygenases (LPMOs) in lignocellulose deconstruction

RR Singhania, P Dixit, AK Patel, BS Giri, CH Kuo… - Bioresource …, 2021 - Elsevier
Lytic polysaccharide monooxygenases (LPMOs) emerged a decade ago and have been
described as biomass deconstruction boosters as they play an extremely important role in …

Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

H Chang, N Gacias Amengual, A Botz… - Nature …, 2022 - nature.com
Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing
saccharification efficiency and rate. Recent studies demonstrate that H2O2 rather than O2 is …

A conserved second sphere residue tunes copper site reactivity in lytic polysaccharide monooxygenases

KR Hall, C Joseph, I Ayuso-Fernández… - Journal of the …, 2023 - ACS Publications
Lytic polysaccharide monooxygenases (LPMOs) are powerful monocopper enzymes that
can activate strong C–H bonds through a mechanism that remains largely unknown. Herein …

The “life-span” of lytic polysaccharide monooxygenases (LPMOs) correlates to the number of turnovers in the reductant peroxidase reaction

S Kuusk, VGH Eijsink, P Väljamäe - Journal of Biological Chemistry, 2023 - ASBMB
Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that degrade the
insoluble crystalline polysaccharides cellulose and chitin. Besides the H 2 O 2 cosubstrate …

Enhanced in situ H2O2 production explains synergy between an LPMO with a cellulose-binding domain and a single-domain LPMO

AA Stepnov, VGH Eijsink, Z Forsberg - Scientific Reports, 2022 - nature.com
Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that catalyze
oxidative depolymerization of recalcitrant substrates such as chitin or cellulose. Recent work …

Expanding the catalytic landscape of metalloenzymes with lytic polysaccharide monooxygenases

A Munzone, VGH Eijsink, JG Berrin… - Nature Reviews …, 2024 - nature.com
Lytic polysaccharide monooxygenases (LPMOs) have an essential role in global carbon
cycle, industrial biomass processing and microbial pathogenicity by catalysing the oxidative …

On the impact of carbohydrate-binding modules (CBMs) in lytic polysaccharide monooxygenases (LPMOs)

Z Forsberg, G Courtade - Essays in Biochemistry, 2023 - portlandpress.com
Lytic polysaccharide monooxygenases (LPMOs) have revolutionized our understanding of
how enzymes degrade insoluble polysaccharides. Compared with the substantial …

Kinetic characterization of a putatively chitin-active LPMO reveals a preference for soluble substrates and absence of monooxygenase activity

L Rieder, D Petrović, P Valjamae, VGH Eijsink… - ACS …, 2021 - ACS Publications
Enzymes known as lytic polysaccharide monooxygenases (LPMOs) are recognized as
important contributors to aerobic enzymatic degradation of recalcitrant polysaccharides such …

Insights into the H2O2‐driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

TM Hedison, E Breslmayr, M Shanmugam… - The FEBS …, 2021 - Wiley Online Library
Fungal lytic polysaccharide monooxygenases (LPMOs) depolymerise crystalline cellulose
and hemicellulose, supporting the utilisation of lignocellulosic biomass as a feedstock for …