The alpha-fetoprotein third domain receptor binding fragment: in search of scavenger and associated receptor targets

GJ Mizejewski - Journal of Drug Targeting, 2015 - Taylor & Francis
Recent studies have demonstrated that the carboxyterminal third domain of alpha-
fetoprotein (AFP-CD) binds with various ligands and receptors. Reports within the last …

Engineering of the Saccharomyces cerevisiae yeast strain with multiple chromosome-integrated genes of human alpha-fetoprotein and its high-yield secretory …

E Dudich, I Dudich, L Semenkova… - Protein expression and …, 2012 - Elsevier
Alpha-fetoprotein (AFP) is a biological drug candidate of high medicinal potential in the
treatment of autoimmune diseases, cancer, and regenerative medicine. Large-scale …

Recombinant alpha-fetoprotein receptor-binding domain co-expression with polyglutamate tags facilitates in vivo folding in E. coli

M Mollaev, N Gorokhovets, E Nikolskaya… - Protein Expression and …, 2018 - Elsevier
A wide range of methods are known to increase the prokaryotic intracellular recombinant
proteins solubility, for instance, growth at low temperature, supplementation of culture media …

The receptor binding fragment of alpha-fetoprotein is a promising new vector for the selective delivery of antineoplastic agents

GA Posypanova, VA Makarov… - Journal of Drug …, 2013 - Taylor & Francis
The alpha-fetoprotein (AFP) binding protein, a putative AFP receptor, is a tumour marker that
is present on the surfaces of malignant cells. AFP enters cells through receptor-mediated …

Efficient refolding of a hydrophobic protein with multiple S–S bonds by on-resin immobilized metal affinity chromatography

OA Sharapova, MS Yurkova… - … of Chromatography A, 2011 - Elsevier
The efficient refolding of recombinant proteins produced in the form of inclusion bodies (IBs)
in Escherichia coli still is a complicated experimental problem especially for large …

Alpha-fetoprotein and recombinant alpha-fetoprotein fragments as drug delivery tools

G Posypanova, S Severin - Alpha-fetoprotein: Functions and Clinical …, 2016 - elibrary.ru
This chapter deals with the problem of developing selectively acting antitumor agents in the
form of bifunctional molecules and complexes. Such constructs comprise a targeting (vector) …

Recombinant human alpha-fetoprotein as a regulator of adipose tissue stromal cell activity

ES Zubkova, LN Semenkova, IV Dudich… - Russian Journal of …, 2012 - Springer
Recombinant human alpha-fetoprotein (rhAFP), expressed in the yeast system as a
glycoprotein, has been isolated and purified to 98% by the multistep method. The testing of …

Рекомбинантный человеческий альфа-фетопротеин как регулятор активности стромальных клеток жировой ткани

ЕС Зубкова, ЛН Семенкова, ИВ Дудич… - Биоорганическая …, 2012 - elibrary.ru
Рекомбинантный альфа-фетопротеин человека (rhAFP), экспрессирующийся в
дрожжевой системе как гликопротеин, получен и очищен по разработанной …

Physicochemical Characteristics of a Variant of Chaperon GroEL Apical Domain Designed to Enhance the Expression and Stability of Target Proteins

KA Kurov, OI Savvin, MS Yurkova, VA Zenin… - Applied biochemistry …, 2019 - Springer
This work describes the properties of a new protein, a modification of GroEL apical domain
designed to be a leader in fusion systems. This polypeptide leader demonstrates a high …

[引用][C] 甲胎蛋白的原核表达及复性优化

才蕾, 矫丽媛, 王继华, 唐时幸 - 生物技术通报, 2015