Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Ångström-resolution fluorescence microscopy

SCM Reinhardt, LA Masullo, I Baudrexel, PR Steen… - Nature, 2023 - nature.com
Fluorescence microscopy, with its molecular specificity, is one of the major characterization
methods used in the life sciences to understand complex biological systems. Super …

Build and operation of a custom 3D, multicolor, single-molecule localization microscope

RM Power, A Tschanz, T Zimmermann, J Ries - Nature Protocols, 2024 - nature.com
Single-molecule localization microscopy (SMLM) enables imaging scientists to visualize
biological structures with unprecedented resolution. Particularly powerful implementations of …

Clathrin coats partially preassemble and subsequently bend during endocytosis

M Mund, A Tschanz, YL Wu, F Frey, JL Mehl… - Journal of Cell …, 2023 - rupress.org
Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular
cargo. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains …

Global fitting for high-accuracy multi-channel single-molecule localization

Y Li, W Shi, S Liu, I Cavka, YL Wu, U Matti… - Nature …, 2022 - nature.com
Multi-channel detection in single-molecule localization microscopy greatly increases
information content for various biological applications. Here, we present globLoc, a graphics …

Particle fusion of super-resolution data reveals the unit structure of Nup96 in Nuclear Pore Complex

W Wang, A Jakobi, YL Wu, J Ries, S Stallinga… - Scientific Reports, 2023 - nature.com
Single molecule localization microscopy offers resolution nearly down to the molecular level
with specific molecular labelling, and is thereby a promising tool for structural biology. In …

AI analysis of super-resolution microscopy: Biological discovery in the absence of ground truth

IR Nabi, B Cardoen, IM Khater, G Gao, TH Wong… - Journal of Cell …, 2024 - rupress.org
Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular
localization tools to study molecular structure at the nanoscale level in the intact cell …

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization

S Liu, J Chen, J Hellgoth, LR Müller, B Ferdman… - Biophysical …, 2024 - cell.com
1617-Pos Contribution of desmoplakin isoforms to desmosome architecture Krishna A.
Patel, Collin M. Ainslie, Alexa L. Mattheyses. Department of Cell, Developmental and …

Machine Learning for Single-Molecule Localization Microscopy: From Data Analysis to Quantification

J Liu, Y Li, T Chen, F Zhang, F Xu - Analytical Chemistry, 2024 - ACS Publications
Single-molecule localization microscopy (SMLM) is a versatile tool for realizing nanoscale
imaging with visible light and providing unprecedented opportunities to observe …

Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis

M Mund, A Tschanz, YL Wu, F Frey, JL Mehl… - BioRxiv, 2021 - biorxiv.org
Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular
cargos. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains …