Ending all forms of hunger by 2030, as set forward in the UN-Sustainable Development Goal
2 (UN-SDG2), is a daunting but essential task, given the limited timeline ahead and the …

Advancements in Genome editing technologies have revolutionized the fields of functional
genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short …

Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome
editing system are usually mosaics in the T1 generation. In this study, we used egg cell …

Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …

Tomato is the highest value fruit and vegetable crop worldwide, yet produces α-tomatine, a
renowned toxic and bitter-tasting anti-nutritional steroidal glycoalkaloid (SGA) involved in …

Paclitaxel is an anticancer therapeutic produced by the yew tree. Over the last two decades,
a significant bottleneck in the reconstitution of early paclitaxel biosynthesis has been the …

Carotenoids are vital phytonutrients widely recognised for their health benefits. Therefore, it
is vital to thoroughly investigate the metabolic regulatory network underlying carotenoid …

Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for …

The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Building expression constructs for transgenesis is one of the fundamental day-to-day tasks
in modern biology. Traditionally it is based on a multitude of type II restriction endonucleases …