[HTML][HTML] Vero cell upstream bioprocess development for the production of viral vectors and vaccines

S Kiesslich, AA Kamen - Biotechnology advances, 2020 - Elsevier
The Vero cell line is considered the most used continuous cell line for the production of viral
vectors and vaccines. Historically, it is the first cell line that was approved by the WHO for the …

Optimization of physical parameters for cell attachment and growth on macroporous microcarriers

YC Ng, JM Berry, M Butler - Biotechnology and bioengineering, 1996 - Wiley Online Library
The rates of cell attachment of the anchorage‐dependent mammalian cell line Vero to the
gelatin‐based macroporous microcarrier Cultispher‐G were determined under various …

High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture

CC Liu, WC Lian, M Butler, SC Wu - Vaccine, 2007 - Elsevier
Developing an effective vaccine against enterovirus 71 (EV71) infection provides the best
means to control the disease. We have previously reported that large-scale preparation of a …

Vero-cell rabies vaccine produced using serum-free medium

NM Frazatti-Gallina, RM Mourão-Fuches, RL Paoli… - Vaccine, 2004 - Elsevier
A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in
a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain …

Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development

SC Wu, CC Liu, WC Lian - Vaccine, 2004 - Elsevier
Enterovirus 71 (EV71) is an enterovirus that could lead to severe neurological disorders and
fatalities. The inactivated vaccine is an appropriate EV71 vaccine format for meeting current …

Optimization of virus yield as a strategy to improve rabies vaccine production by Vero cells in a bioreactor

K Trabelsi, S Rourou, H Loukil, S Majoul… - Journal of biotechnology, 2006 - Elsevier
To improve rabies vaccine production by Vero cells, we have developed a strategy based on
high cell density culture and optimization of virus yield. We have first optimized cell growth in …

Application of a serum‐free medium for the growth of Vero cells and the production of reovirus

M Butler, A Burgener, M Patrick, M Berry… - Biotechnology …, 2000 - Wiley Online Library
Two strains of reovirus (serotype 1 Lang/TIL and serotype 3 Dearing/T3D) were propagated
in Vero cells grown in stationary or agitated cultures in a serum‐free medium, M‐VSFM …

Production of retrovirus and adenovirus vectors for gene therapy: a comparative study using microcarrier and stationary cell culture

SC Wu, GYL Huang, JH Liu - Biotechnology progress, 2002 - Wiley Online Library
In gene therapy, retrovirus and adenovirus vectors are extensively used as gene‐delivery
vehicles and further large‐scale processing of these viral vectors will be increasingly …

Viral vaccine production in cell culture

JG Auniņš - Encyclopedia of industrial biotechnology …, 2009 - Wiley Online Library
Cell culture for the purposes of viral vaccine cultivation is reviewed from the perspective of
the industrial practitioner. This article covers the mechanics of virus production, followed by …

Higher production of rabies virus in serum-free medium cell cultures on microcarriers

NM Frazzati-Gallina, RL Paoli… - Journal of …, 2001 - Elsevier
Rabies virus suspensions were obtained from VERO cells cultivated on solid microcarriers
in a bioreactor after infection with the Pasteur rabies virus strain (PV). Virus production …