Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions
RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos… - Biotechnology …, 2019 - Elsevier
Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
Isocyanide-based multi-component reactions for carrier-free and carrier-bound covalent immobilization of enzymes
Strategies for the covalent immobilization of enzymes depend on the type of functional group
selected to perform the coupling reaction, and on the relative importance of selectivity …
selected to perform the coupling reaction, and on the relative importance of selectivity …
Process optimization for biodiesel production from waste cooking oil using multi-enzyme systems through response surface methodology
Lipase from Rhizomucor miehei (RML) and lipase B from Candida antarctica (CALB) were
covalently immobilized onto epoxy-functionalized silica. In this study, we developed a multi …
covalently immobilized onto epoxy-functionalized silica. In this study, we developed a multi …
Amination of enzymes to improve biocatalyst performance: Coupling genetic modification and physicochemical tools
Improvement of the features of an enzyme is in many instances a pre-requisite for the
industrial implementation of these exceedingly interesting biocatalysts. To reach this goal …
industrial implementation of these exceedingly interesting biocatalysts. To reach this goal …
Hyperactivation of lipases by immobilization on superhydrophobic graphene quantum dots inorganic hybrid nanoflower
M Mostafavi, MB Poor, Z Habibi, M Mohammadi… - International Journal of …, 2024 - Elsevier
Various nanoflowers are synthesized for enzyme immobilization. In order to increase the
activity of nanoflowers, in this study, 3D flower-like structured organic–inorganic hybrid …
activity of nanoflowers, in this study, 3D flower-like structured organic–inorganic hybrid …
Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil
In the present work, lipases from Candida antarctica (CALB), Thermomyces lanuginosus
(TLL) and Rhizomucor miehei (RML) were covalently immobilized on epoxy-functionalized …
(TLL) and Rhizomucor miehei (RML) were covalently immobilized on epoxy-functionalized …
Polyethyleneimine-modified superparamagnetic Fe3O4 nanoparticles for lipase immobilization: Characterization and application
Magnetically separable nanospheres consisting of polyethyleneimine (PEI) and succinated
PEI grafted on silica coated magnetite (Fe 3 O 4) were prepared and characterized using …
PEI grafted on silica coated magnetite (Fe 3 O 4) were prepared and characterized using …
Application of multi-component reaction for covalent immobilization of two lipases on aldehyde-functionalized magnetic nanoparticles; production of biodiesel from …
M Ashjari, M Garmroodi, FA Asl, M Emampour… - Process …, 2020 - Elsevier
Lipase from Rhizomucor miehei (RML) and Thermomyces lanuginosa lipase (TLL) were
immobilized on silica core-shell magnetic nanoparticles (Fe 3 O 4@ SiO 2) produced by …
immobilized on silica core-shell magnetic nanoparticles (Fe 3 O 4@ SiO 2) produced by …
Lipase immobilization onto polyethylenimine coated magnetic nanoparticles assisted by divalent metal chelated ions
In this study, polyethylenimine coated Fe 3 O 4 magnetic nanoparticles (Fe@ PEI) were
prepared and used for three metal ions (Co 2+, Cu 2+ and Pd 2+) chelation. The metal …
prepared and used for three metal ions (Co 2+, Cu 2+ and Pd 2+) chelation. The metal …
Covalent binding of hyper-activated Rhizomucor miehei lipase (RML) on hetero-functionalized siliceous supports
M Garmroodi, M Mohammadi, A Ramazani… - International journal of …, 2016 - Elsevier
Physical adsorption onto hydrophobic supports has proven to be an effective way to improve
the activity of lipases. Covalent binding, on the other hand, enhances the active lifetime of …
the activity of lipases. Covalent binding, on the other hand, enhances the active lifetime of …