The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate
the activity of genes in eukaryotic cells in diverse settings, varying from basic biological …

Virus vaccines have to be immunogenic, sufficiently stable, safe, and suitable to induce long-
lasting immunity. To meet these requirements, vaccine studies need to provide a …

Background Hepatocellular carcinoma (HCC) is one of the most common malignancies
worldwide, and tumor recurrence and metastasis are major factors that contribute to the poor …

TET2 is frequently mutated in myeloid neoplasms. Genetic TET2 deficiency leads to skewed
myeloid differentiation and clonal expansion, but minimal residual TET activity is critical for …

Molecular recognition by RNA aptamers has been exploited to control gene expression in
response to small molecules in mammalian cells. These mammalian synthetic riboswitches …

Conditional gene expression systems that enable inducible and reversible transcriptional
control are essential research tools and have broad applications in biomedicine and …

Self-amplifying RNA replicons are promising platforms for vaccine generation. Their defects
in one or more essential functions for viral replication, particle assembly, or dissemination …

Background Brain microvascular endothelial cells (BMECs) play a major role in the blood–
brain barrier (BBB), and are critical for establishing an in vitro BBB model. Currently, iPSC …

With the ability to affect multiple genes and fundamental pathways simultaneously, miRNA
engineering of Chinese Hamster Ovary (CHO) cells has significant advantages over single …

The production of recombinant proteins for functional and biophysical studies, especially in
the field of structural determination, still represents a challenge as high quality and …