Highlights•Ligand-responsive biosensors are integral to cellular-control applications.•
Integrated biosensors connect ligand recognition to a single output signal.•Portable …

Chemical biosensors are an increasingly ubiquitous part of our lives. Beyond enzyme-
coupled assays, recent synthetic biology advances now allow us to hijack more complex …

The optimization of cellular functions often requires the balancing of gene expression, but
the physical construction and screening of alternative designs are costly and time …

Modules, toolboxes, and synthetic biology systems may be designed to address
environmental bioremediation. However, weak and decentralized functional modules …

High-throughput (HT) methodologies are extensively applied in synthetic biology for the
rapid enrichment and selection of desired properties from a wide range of genetic diversity …

Highlights•Engineering for the bioproduction of natural products is bottlenecked by
screening.•To overcome this, we explore advancements in biosensors and analytical …

Aromatic amino acids (AAA) and derived compounds have enormous commercial value with
extensive applications in the food, chemical and pharmaceutical fields. Microbial production …

Prokaryotic transcription factors (TFs) regulate gene expression in response to small
molecules, thus representing promising candidates as versatile small molecule‐detecting …

Transcription factor (TF)-based biosensors are useful synthetic biology tools for applications
in a variety of areas of biotechnology. A major challenge of biosensor circuits is the limited …

Background The efficient utilization of natural regulatory networks offers a practical strategy
for optimizing the production of crucial bio-chemicals. In recent years, acid resistance has …