Highlights•Halogenation is a powerful strategy to tune physicochemical features of organic
molecules.•Synthetic metabolism can be tailored for the incorporation of halogen …

Lignocellulose is a promising feedstock for biofuel production as a renewable, carbohydrate-
rich and globally abundant source of biomass. However, challenges faced include …

Metabolic engineering (ME) aims to develop efficient microbial cell factories that can
produce a wide variety of valuable compounds, ideally at the highest yield and from various …

Anaerobic fungi and methanogenic archaea are two classes of microorganisms found in the
rumen microbiome that metabolically interact during lignocellulose breakdown. Here, stable …

Lignocellulosic biorefineries require innovative solutions to realize their full potential, and
the discovery of novel lignocellulose-active enzymes could improve biorefinery …

In the yeast Saccharomyces cerevisiae, microbial fuels and chemicals production on
lignocellulosic hydrolysates is constrained by poor sugar transport. For biotechnological …

Anaerobic fungi are powerful platforms for biotechnology that remain unexploited due to a
lack of genetic tools. These gut fungi encode the largest number of lignocellulolytic …

Biocontainment systems are needed to neutralize genetically modified organisms (GMOs)
that pose ecological threats outside of controlled environments. In contrast, benign selection …

Anaerobic fungi (Neocallimastigomycota) are emerging non-model hosts for biotechnology
due to their wealth of biomass-degrading enzymes, yet tools to engineer these fungi have …

Membrane‐embedded transporters impart essential functions to cells as they mediate
sensing and the uptake and extrusion of nutrients, waste products, and effector molecules …