[HTML][HTML] The CRISPR/Cas9 system for plant genome editing and beyond

L Bortesi, R Fischer - Biotechnology advances, 2015 - Elsevier
Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

Genetic engineering for disease resistance in plants: recent progress and future perspectives

OX Dong, PC Ronald - Plant physiology, 2019 - academic.oup.com
Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future
Perspectives | Plant Physiology | Oxford Academic Skip to Main Content Advertisement …

Prime genome editing in rice and wheat

Q Lin, Y Zong, C Xue, S Wang, S Jin, Z Zhu… - Nature …, 2020 - nature.com
Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions
programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable …

S Schiml, F Fauser, H Puchta - The Plant Journal, 2014 - Wiley Online Library
The CRISPR/C as nuclease is becoming a major tool for targeted mutagenesis in
eukaryotes by inducing double‐strand breaks (DSB s) at pre‐selected genomic sites that are …

Targeted DNA insertion in plants

OX Dong, PC Ronald - … of the National Academy of Sciences, 2021 - National Acad Sciences
Conventional methods of DNA sequence insertion into plants, using Agrobacterium-
mediated transformation or microprojectile bombardment, result in the integration of the DNA …

Genome editing for plant research and crop improvement

X Zhan, Y Lu, JK Zhu, JR Botella - Journal of Integrative Plant …, 2021 - Wiley Online Library
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

[HTML][HTML] Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system

RF Xu, H Li, RY Qin, J Li, CH Qiu, YC Yang, H Ma… - Scientific reports, 2015 - nature.com
The CRISPR/Cas9 system is becoming an important genome editing tool for crop breeding.
Although it has been demonstrated that target mutations can be transmitted to the next …

CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination

TK Huang, H Puchta - Plant Cell Reports, 2019 - Springer
Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

Synthetic nucleases for genome engineering in plants: prospects for a bright future

H Puchta, F Fauser - The Plant Journal, 2014 - Wiley Online Library
By inducing double‐strand breaks (DSB), it is possible to initiate DNA recombination. For a
long time, it was not possible to use DSB induction for efficient genome engineering due to …