Virus-induced gene silencing: empowering genetics in non-model organisms

AB Dommes, T Gross, DB Herbert… - Journal of …, 2019 - academic.oup.com
Virus-induced gene silencing (VIGS) is an RNA interference-based technology used to
transiently knock down target gene expression by utilizing modified plant viral genomes …

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

T Li, J Hu, Y Sun, B Li, D Zhang, W Li, J Liu, D Li… - Molecular Plant, 2021 - cell.com
Genome editing provides novel strategies for improving plant traits but mostly relies on
conventional plant genetic transformation and regeneration procedures, which can be …

A barley stripe mosaic virus‐based guide RNA delivery system for targeted mutagenesis in wheat and maize

J Hu, S Li, Z Li, H Li, W Song, H Zhao… - Molecular Plant …, 2019 - Wiley Online Library
Plant RNA virus‐based guide RNA (gRNA) delivery has substantial advantages compared
to that of the conventional constitutive promoter‐driven expression due to the rapid and …

Coat proteins of necroviruses target 14-3-3a to subvert MAPKKKα-mediated antiviral immunity in plants

Z Gao, D Zhang, X Wang, X Zhang, Z Wen… - Nature …, 2022 - nature.com
Mitogen-activated protein kinase (MAPK) cascades play an important role in innate immunity
against various pathogens in plants and animals. However, we know very little about the …

Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data …

P Sudhakar Reddy, D Srinivas Reddy… - Frontiers in plant …, 2016 - frontiersin.org
Accurate and reliable gene expression data from qPCR depends on stable reference gene
expression for potential gene functional analyses. In this study, 15 reference genes were …

The dynamics of N6-methyladenine RNA modification in interactions between rice and plant viruses

K Zhang, X Zhuang, Z Dong, K Xu, X Chen, F Liu, Z He - Genome Biology, 2021 - Springer
Abstract Background N 6-methyladenosine (m6A) is the most common RNA modification in
eukaryotes and has been implicated as a novel epigenetic marker that is involved in various …

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR

H Ling, Q Wu, J Guo, L Xu, Y Que - PloS one, 2014 - journals.plos.org
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method
for gene expression analysis requires one or several reference gene (s) acting as …

Evaluation of transcription factor and aquaporin gene expressions in response to Al2O3 and ZnO nanoparticles during barley germination

H Akdemir - Plant Physiology and Biochemistry, 2021 - Elsevier
Aluminum oxide and zinc oxide nanoparticles (NPs) are two of the mostly produced
engineered metal oxide NPs. Here, barley germination and root elongation as well as gene …

Isolation, structural characterization and biological activities of polysaccharides from Chondrus crispus

AD Premarathna, A Sooäär, TAE Ahmed, V Rjabovs… - Food …, 2024 - Elsevier
Biomedical applications often exploit the biodegradability and biocompatibility of natural
polysaccharides. In this study, polysaccharide extracts from Chondrus crispus (CC) were …

Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses

P Shukla, RA Reddy, KM Ponnuvel, GK Rohela… - Molecular biology …, 2019 - Springer
Mulberry (Morus alba L.) is the sole food source for the mulberry silkworm, Bombyx mori and
therefore important for sericulture industry. Different abiotic stress conditions like drought …