Structures and general transport mechanisms by the major facilitator superfamily (MFS)

D Drew, RA North, K Nagarathinam… - Chemical reviews, 2021 - ACS Publications
The major facilitator superfamily (MFS) is the largest known superfamily of secondary active
transporters. MFS transporters are responsible for transporting a broad spectrum of …

Paramagnetic chemical probes for studying biological macromolecules

Q Miao, C Nitsche, H Orton, M Overhand… - Chemical …, 2022 - ACS Publications
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Lipid membrane mimetics in functional and structural studies of integral membrane proteins

S Majeed, AB Ahmad, U Sehar, ER Georgieva - Membranes, 2021 - mdpi.com
Integral membrane proteins (IMPs) fulfill important physiological functions by providing cell–
environment, cell–cell and virus–host communication; nutrients intake; export of toxic …

Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins: CuII–CuII and Nitroxide–Nitroxide Cases

K Ackermann, JL Wort, BE Bode - The Journal of Physical …, 2021 - ACS Publications
The study of ever more complex biomolecular assemblies implicated in human health and
disease is facilitated by a suite of complementary biophysical methods. Pulse dipolar …

Structure and mechanism of membrane transporters

L Guan - Scientific Reports, 2022 - nature.com
Membrane transporters are an important group of proteins in physiology and disease. Their
functions make them common drug targets, but their location in the lipid bilayers poses a …

A general model to optimise Cu II labelling efficiency of double-histidine motifs for pulse dipolar EPR applications

JL Wort, K Ackermann, DG Norman… - Physical Chemistry …, 2021 - pubs.rsc.org
Electron paramagnetic resonance (EPR) distance measurements are making increasingly
important contributions to studies of biomolecules underpinning health and disease by …

Molecular Dynamics Simulations Based on Newly Developed Force Field Parameters for Cu2+ Spin Labels Provide Insights into Double-Histidine-Based Double …

X Bogetti, S Ghosh, A Gamble Jarvi… - The Journal of …, 2020 - ACS Publications
Electron paramagnetic resonance (EPR) in combination with the recently developed double-
histidine (dHis)-based Cu2+ spin labeling has provided valuable insights into protein …

Characteristics of Gd (III) spin labels for the study of protein conformations

A Giannoulis, Y Ben-Ishay, D Goldfarb - Methods in enzymology, 2021 - Elsevier
Gd (III) complexes are currently established as spin labels for structural studies of
biomolecules using pulse dipolar electron paramagnetic resonance (PD-EPR) techniques …

Triggered Functional Dynamics of AsLOV2 by Time‐Resolved Electron Paramagnetic Resonance at High Magnetic Fields

S Maity, BD Price, CB Wilson, A Mukherjee… - Angewandte …, 2023 - Wiley Online Library
We present time‐resolved Gd− Gd electron paramagnetic resonance (TiGGER) at 240 GHz
for tracking inter‐residue distances during a protein's mechanical cycle in the solution state …

A Comparative Study of Nitroxide‐Based Biradicals for Dynamic Nuclear Polarization in Cellular Environments

BE Ackermann, BJ Lim, N Elathram… - …, 2022 - Wiley Online Library
Dynamic nuclear polarization (DNP) is a powerful tool to enhance the NMR signals of
molecules by transferring polarization from unpaired electron spins to nuclei through …